膀胱移行细胞和移行细胞癌细胞内β-葡萄糖醛酸酶免疫电镜比较研究  

A COMPARATIVE STUDY OF THE IMMUNOELECTRON MICROSCOPE OF β GLUCURONIDASE BETWEEN TRANSITIONAL CELLS AND TRANSITIONAL CELL CARCINOMA OF BLADDER

作　　者：张弘[1,2] 杨波[1,2] 于安民[1,2] 宋继谒 

机构地区：[1]中国医科大学病理解剖学教研室 [2]沈阳军区总医院

出　　处：《中国组织化学与细胞化学杂志》1997年第3期115-118,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：β－葡萄糖醛酸酶（β－Ｇｌｕｃｕｒｏｎｉｄａｓｅ，简称β－Ｇ）在正常人体组织匀浆和体液中含量很低，本实验采用胶体金标记，免疫电镜技术，进行了人体正常移行细胞与移行细胞癌细胞内β－Ｇ定位研究，实验结果表明，β－Ｇ存在于移行细胞和移行细胞癌细胞中的内质网、溶酶体内，同时观察到癌细胞中标记β－Ｇ的金颗粒数量多于正常移行细胞中金颗粒的数量，本实验结果可能对于移行细胞癌的早期发现、早期诊断提供了新的依据。β Glucuronidase(β G) is low in normal human tissue fragment and body liquid. In this experiment, colloid gold labelling and immunoelectron microscope technique were used for the localization of β G in normal human body transitional cell and transitional cell carcinoma respectively. The result showed that β G was localized in endoplasmic reticulum and lysosome in normal and malignant transitional cells. At the same time, we found that the number of gold granules was greater in carcinoma cells than in normal transitional ones. The result of the experiment may offer a new method for early discovering and early diagnosing of transitional cell carcinoma.

关 键 词：Β-葡萄糖醛酸酶 移行细胞 移行细胞癌 胶体金 免疫电镜 

分 类 号：Q55[生物学—生物化学]