固定对免疫组织化学反应的影响  

THE INFLUENCE OF FIXATIVES ON IMMUNOHISTOCHEMISTRY

作　　者：古德全[1] 

机构地区：[1]第三军医大学防原医学教研室

出　　处：《中国组织化学与细胞化学杂志》1997年第1期80-83,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：为在组织固定过程中最大程度地减少固定剂对抗原性和组织结构的破坏，使免疫组织化学反应清晰可靠，我们观察研究了１０％中性缓冲福尔马林（ＮＢＦ）、酒精－甲醛（ＡＦ）、甲醛钙（Ｆｃａ）、Ｃａｒｎｏｙ氏液、Ｂｏｕｉｎ氏液、Ｋａｒｎｏｖｓｋｙ氏液和Ｈｅｌｙ氏液，分别对ＡＢＣ法显示基底膜Ⅳ型胶原，ＳＰ法显示血管内皮第Ⅷ因子和小肠固有层浆细胞的影响。结果表明用同一免疫组织化学方法显示不同固定剂固定的同一抗原物质可出现明显差异。本文还简要阐述了有关固定剂的作用机理与免疫组织化学染色的相关性。为观察研究组织和细胞的某种或某些抗原物质。In order to reduce as much as possible the destruction of fixatives on antigenicity and tissue structures in the course of tissue fixation and to obtain a clear and more reliable immunohistochemical stain, we studied the influences of 10% neutral buffered formalin (NBF), alcohol-formalidehyde (AF), formalin-calcium(FCa), Bouins solution, Carnoys solution, Karnovskys solution and Hellys solution on the staining effects of ABC method to show the basement membranes and of SP method to show factorⅧ and plasma cells. The results showed that, by using the same immunohistochemical method for demonstrating the same antigen in different fixation, the staining effect varies greatly. This article has discussed concisely the relationship between the mechanism of fixatives and immunohistochemical stain. This will provide a scientific base for selection of suitable fixatives.

关 键 词：固定剂 免疫组织化学 

分 类 号：R392.11[医药卫生—免疫学]