小鼠胸腺哺育细胞内淋巴细胞碱性磷酸酶电镜观察  

ELECTRON MICROSCOPIC OBSERVATION OF ALKALINE PHOSPHATASE OF LYMPHOCYTE WITHIN THYMIC NURSE CELL (TNC) IN MICE

作　　者：毛曦[1,2] 缪继武[1,2] 尹兰珍 

机构地区：[1]江苏职工医科大学 [2]南京铁道医学院

出　　处：《中国组织化学与细胞化学杂志》1997年第1期89-92,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：碱性磷酸酶（ａｌｋａｌｉｎｅｐｈｏｓｐｈａｔａｓｅ，ＡＬＰ）是研究Ｔ淋巴细胞分裂分化的标记之一。胸腺内淋巴母细胞具有膜性ＡＬＰ。本文采用ＡＬＰ超微结构细胞化学方法，探讨小鼠胸腺哺育细胞（Ｔｈｙｍｉｃｎｕｒｓｅｃｅｌ，ＴＮＣ）内淋巴细胞（ＴＮＣ－Ｌ）ＡＬＰ的活性分布。观察结果，２０％的ＴＮＣ内有少数淋巴细胞呈阳性反应，而ＴＮＣ外游离的淋巴细胞均为ＡＬＰ阴性反应。结果表明，部分ＴＮＣ内含有少量淋巴母细胞亚群。Alkaline phosphatase(ALP) is one of the markers used to study the division and differentiation of T Lymphocyte. The lymphoblasts in thymus possess membrane ALPase activity. In our studies the distribution of ALPase activity of lymphocytes within thymic nurse cell(TNC-L) was investigated by means of an ultrastructural cytochemical method. The observation revealed that within 20% TNC-L a small number of lymphocytes showed ALPase-positive activity, however, the free lymphocytes outside TNC appeared as the negative reaction. The result indicates that there are a few subpopulations of lymphoblast in parts of TNC and TNC is one of the microenvironments for the Tlymphocyte division and differentiation.

关 键 词：胸腺哺育细胞 淋巴细胞 碱性磷酸酶 小鼠 电镜 

分 类 号：Q55[生物学—生物化学]