培养骨髓基质细胞的尿嘧啶核苷二磷酸半乳糖-4-表异构酶活性  

OBSERVATION OF THE URIDINE DIPHOSPHOGALACTOSE-4-EPIMERASE ACTIVITY OF BONE MARROW STROMAL CELLS IN CULTURE

作　　者：陈春华[1,2] 苏秀兰[1,2] 舍英[1,2] 张立中 

机构地区：[1]内蒙古医学院细胞化学研究室 [2]内蒙古自治区医院

出　　处：《中国组织化学与细胞化学杂志》1997年第1期113-116,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：本实验用液体静置法体外培养小鼠骨髓基质细胞。用细胞化学法观察处于不同分化阶段基质细胞中的尿嘧啶核苷二磷酸半乳糖－－４－表异构酶（Ｕｒｉｄｉｎｅｄｉｐｈｏｓｐｈｏｇａｌａｃｔｏｓｅ－４－ｅｐｉｍｅｒａｓｅ，ＵＤＰＧａｌ，ＥＣ５．１．３．ａ）活性。结果在各类骨髓基质细胞中均可见到很强的酶反应。在由原始网状细胞向成熟星状细胞分化过程中，酶活性逐渐增强。随着培养时间和延长（培养５、７、１０天），星状细胞和成纤维细胞中酶反应的阳性率和阳性度逐渐增高。Bone marrow stromal cell was cultured in stationary liquid and the activity of UDPGal was observed for distinct differentiation stage by cytochemical method. The results showed that there was very strong enzyme reaction in various bone marrow stromal cells. From original reticulated cells differentiation to mature stellate cells, the enzyme activity was increased gradually. With the prolonging of culture time (5d, 7d, 10d), positive grade and rate of erzyme reaction in stellate cells and fibroblasts were raised constantly. The result indicated that there was an active metabolism UDPGal catalysis in cultured bone marrow stromal cell.

关 键 词：骨髓——细胞学 细胞化学 表异构酶 

分 类 号：Q26[生物学—细胞生物学]