酶联免疫双抗夹心法测定Ⅳ型胶原蛋白  

Establishment of ELISA for measuring type Ⅳ collagen

作　　者：姚敏捷[1] 黄汉朝[1] 袁忠海[1] 郭素红[1] 刘诗福[1] 

机构地区：[1]空军医学高等专科学校,吉林市132013

出　　处：《空军医高专学报》1997年第2期63-65,共3页

摘　　要：建立血清Ⅳ型胶原蛋白(COL Ⅳ)浓度测定的酶联免疫吸附分析法(ELISA),用以辅助诊断肝硬化。应用此法观察了30例正常人和50例肝纤维化患者,结果显示COL Ⅳ浓度在20μg/L～600μg/L之间时,工作曲线最佳,最低检出限为20μg/L,批内变异系数(CV)6.098％,批间CV4.30％,高、低浓度回收率分别为95％和98％;正常人血清COL Ⅳ平均为86.7μg/L,肝纤维化患者平均水平为260.5μg/L,可见辅助诊断肝纤维化具有较高特异性和灵敏度。To develop ELISA to measure the level of serum type Ⅳ collagen, as an accessaory diagnostic method of hepatocirrhosis. With this method ,30 normal subjects and 50 patients with hepatocirrhosis were observed. The result showed that when the concentration was between 20 μg/L and 600 μg/L,the curve ran the best. The lowest detectable limit was 20 μg/L,the average intra-assay CV was 6. 098 % and the inter-assay CV was 4. 30%. The recovery was 95% in high and 98% in low concentration of typeⅣcollagen. With this assay,the concentration of cypeⅣcollagen averaged 86. 7μg/L in the normal subjects and 260. 5/μg/L in the patients. So, ELISA,as an accessory method,is simple,precise ,specific and low-cost in diagnosis of hepatocirrhosis.

关 键 词：酶联免疫吸附分析法 Ⅳ型胶原 肝纤维化 

分 类 号：R446.6[医药卫生—诊断学]