体外观察MEL细胞系中铁螯合酶mRNA表达的调节  

Expression of Ferrochelatase mRNA in MEL Cell Line In Vitro

作　　者：王伟良[1] 李蓉生[1] 李琦[1] 

机构地区：[1]中国医学科学院,中国协和医科大学北京协和医院血液科,北京100730

出　　处：《中国实验血液学杂志》1996年第3期273-277,共5页Journal of Experimental Hematology

摘　　要：铁螫合酶是参与血红素生物合成过程中的最后一步的酶,催化将铁原子引入卟啉原IX内的反应。它存在于所有的细胞,但在红系细胞内含量尤其丰富。目前已经清楚,铁螫合酶的mRNA有两种形式:2.9kb和2.2kb,其中2.9kb的mRNA主要存在于非红系细胞内,2.2kb的mRNA则主要存在于红系细胞内。采用1.5kbcDNA作为探针,观察小鼠红白血病(murine erythroleukemia,MEL)细胞在二甲基亚枫(DMSO)诱导分化前、后不同的铁状态和不同的血红素合成条件下铁螫合酶mRNA表达的变化。观察结果:①在未诱导的MEL细胞,虽然细胞增生旺盛,但铁螫合酶mRNA表达无变化;②在诱导的MEL细胞,铁螯合酶mRNA表达显著增加;③在诱导和未诱导的MEL细胞,铁对铁螯合酶mRNA表达的影响均较弱;④在诱导的MEL细胞中血红素合成受抑制,铁螯合酶mRNA表达降低。Ferrochelatase, which catalvses the last stop in heme biosynthesis, i. e. the insertion of Fe+ + into protoporphyrin Ⅸ, present in all celis, particularly aboundanty in erythroid cells during hemoglobiniza-tion. In present study, we observed expression of ferrochelatase mRNA in different statuses of intracellular iron and heme synthesis in non- induced and DMSO induced murine erythroleukemia (MEL) celis with 1. 5 kb cDNA as probe. The results were as follows: ① In non - induced celis the expression of ferrochelatase mRNA had no changes. ② The expression of ferrochelatase mRNA had increased in induced celis. ③ The level of intracellular iron in both non - induced and induced cells has effect on ex-pression of ferrochelatase mRNA bt faintful: when iron in excess, the expression of mRNA was increased; when iron in deficiency, the expression was decreased. ④ The inhibition of heme synthesis by INH decreases the expression of ferrochelatase mRNA in induced cells.

关 键 词：铁螫合酶 血红素 铁 MRNA表达 红白血病细胞 

分 类 号：R346[医药卫生—基础医学]