聚乙烯吡咯烷酮接枝壳聚糖的生物相容性评价  

Biocompatibility evaluation of chitosan-g-polyvinylpyrrolidone

作　　者：邓政兴[1] 张志雄[1] 李立华[1] 周长忍[1] 

机构地区：[1]暨南大学生命科技学院,广东广州510632

出　　处：《第一军医大学学报》2004年第6期639-641,645,共4页Journal of First Military Medical University

基　　金：国家自然科学基金(30170272);国家科技部重大基础研究项目(973项目)子课题(G199054306);国家科技部高技术计划项目(863)(2001AA625050)~~

摘　　要：目的 评价聚乙烯吡咯烷酮(PVP)接枝壳聚糖的生物相容性，以制备一种新型的组织工程支架材料。方法 对材料进行接触角、吸水率的测定，并借助体内埋植和细胞培养实验对材料的生物相容性进行系统性评价。结果 接枝后的材料吸水率达1100％，接触角达到83°～86°；体内埋植3个月后材料部分降解，周围组织无明显的炎症反应，角膜上皮细胞在材料上能较好地生长，没有明显的细胞毒性。结论 所制备的PVP接枝壳聚糖具有良好的生物相容性，可作为组织工程支架材料。Objective To evaluate the biocompatibility of chitosan-g-polyvinylpyrrolidone as a new scaffold material. Methods The material was tested and measured for water absorption and contact angle, followed by evaluation of the biocompatibility by implantation into rabbits and in vitro cultured with the corneal epithelial cells. Results The water absorption rate of the material reached 1 100% with contact angle of 83°-86°. The results of implantation revealed partial degradation of the material 3 months after implantation, and much collagen and numerous corneal stromal cells appeared on the material without obvious inflammation reactions. In vitro coculture with epithelial cells showed good adhesion of the cells to the material which induced no obvious cytotoxicity. Conclusion The novel chitosan derivative has excellent biocompatibility and can be used as a tissue scaffold material.

关 键 词：聚乙烯吡略烷酮 壳聚糖 生物相容性 评价 角膜 

分 类 号：R318.08[医药卫生—生物医学工程]