海水型呼吸窘迫综合征犬模型的实验研究  

Respiratory distress syndrome induced by immersion in seawater:a study of canine models

作　　者：傅卫军[1] 古妙宁[1] 陈仲清[1] 肖金仿[1] 黄毅然[1] 周伟[1] 陈晔明[1] 

机构地区：[1]第一军医大学南方医院SICU海水研究组,广东广州510515

出　　处：《第一军医大学学报》2004年第6期665-669,共5页Journal of First Military Medical University

基　　金：广东省自然科学基金(2003C33715)~~

摘　　要：目的构建海水型呼吸窘迫综合征(SW-RDS)的犬模型,为研究海水淹溺后的呼吸窘迫综合征的病理表现和治疗提供平台。方法选择12只健康犬随机分为2组,即对照组(n=4)和模型组(n=8)。对比两组血流动力学、血气分析、电解质等指标和肺组织细胞学变化,并检测支气管肺泡液和血液乳酸脱氢酶(LDH-L)、碱性磷酸酶(ALP)浓度。结果与对照组相比,模型组出现明显的血流动力学、血气和乳酸的变化,病理结果显示肺组织损伤明显,LDH-L和ALP持续显著升高。上述变化符合SW-RDS的诊断标准。结论SW-RDS犬模型能够反映海水淹溺后的病理生理变化规律和SW-RDS的病理特征。Objective To study the mechanism of respiratory distress syndrome (RDS) induced by immersion in seawater to provide experimental evidence for its treatment. Methods Twelve normal hybrid dogs were randomly assigned into control group (n=4) and SDS model group (n=8). The changes in blood dynamics, blood gas analysis and histological changes in the lung tissues were compared between the 2 groups. The concentration of lactic dehydrogenase (LDH-L) and alkaline phospha- tase (ALP) in the bronchoalveolar fluid and blood of the dogs in the model group were tested. Results The blood dynamics, blood gas analysis and histology of the dogs in the model group were significantly different from those in the control group, and LDH-L and ALP levels increased significantly in the bronchoalveolar fluid of the model group. Conclusion Seawater aspiration into the lungs may lead to RDS, and the canine models used in this study may help explore the mechanism and management of RDS induced by immersion in seawater.

关 键 词：呼吸窘迫综合征 动物模型 海水 血气分析 血流动力学 

分 类 号：R563.8[医药卫生—呼吸系统]