机械拉伸对人肺上皮细胞粘附及[Ca^(2+)]i的影响  

Effects of cyclic mechanical stretch on the adhesion and the intracellular calcium concentration of human pulmonary epithelial cells

作　　者：张惠静[1] 秦建[2] 蔡绍皙[2] 卢晓[2] 王远亮[2] 杨力 

机构地区：[1]第三军医大学医学检验系分析化学教研室 [2]重庆大学生物工程学院,生物力学与组织工程教育部重点实验室 [3]Department of Bioengineering and Orthopedics,University of California,SanDiego,CA920930656,USA

出　　处：《第三军医大学学报》2004年第11期953-956,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助重点项目 ( 19732 0 0 3) ;高等学校重点实验室访问学者基金资助项目 ( 2 0 0 2 )~~

摘　　要：目的　探讨周期性拉伸应变对人肺上皮细胞H72 7的力学性质及其 [Ca2 + ]i的调控作用。方法　应用微管吸吮系统测定细胞与基底间的粘附力和细胞的铺展面积 ,用荧光探针Fluo 3 /AM测定拉伸应变对人肺上皮细胞H72 7[Ca2 + ]i的影响。结果　在应变为 15 %,频率为 40次 min的拉伸刺激下 ,与其静态对照组相比 ,细胞与基底间的粘附力和细胞的铺展面积均增加 ;拉伸 5min后 ,[Ca2 + ]i比对照组有明显增加 ,用三七总皂苷阻断胞内Ca2 + 离子通道后 ,细胞对应变的响应仍表现出 [Ca2 + ]i的升高 ,但与未加三七总皂苷的实验组相比 ,[Ca2 + ]i响应应变后的升高由原来的 3 .4倍的增加降低为 1.5倍的增加。结论　在人肺上皮细胞H72 7响应周期性拉伸应变的过程中 ,[Ca2 + ]i的升高既有胞外钙内流的参与 ,也有胞内钙库的释放作用 。Objective To study regulatory effect of cyclic mechanical cyclic stretch on the adhesion and the intracellular calcium concentration of human pulmonary epithelial cells. Methods The micropipette aspiration was used to study the effect of cyclic stretch on the mechanical property of human pulmonary epithelial H727 cells. The fluorescence probe Fluo 3/AM was used to investigate the changes of intracellular calcium concentration when human pulmonary epithelial cells responded to the stretch. Results The cyclic stretch increased the adhesion force, spreading areas, and the intracellular calcium concentration of human pulmonary epithelial cells. Conclusion The rise of intracellular calcium concentration is resulted from the influx of extracellular calcium and the release of intracellular calcium store, in which the influx of extracellular calcium plays the main role.

关 键 词：拉伸 人肺上皮细胞 粘附 胞内钙 

分 类 号：R322.35[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]