家蚕微粒子病血清学检验技术的研究 Ⅲ—N.b碳素凝集反应用于原种母蛾检微  

STUDIES ON SEROLOGLCAL INSPECTION TECHNIQUES FOR NOSEMA BOMBYCLS Ⅲ—N.b-CARBON AGGLUTINATION METHOD FOR THE EXAMINATION OF STOCK FEMALE MOTHS OF SLLKWORM

作　　者：陈祖佩[1] 冯丽春[1] 潘敏慧[1] 万永继[1] 祝仁英[1] 邹康 张明阶 尹泽佑 许毅 刘晓平 

机构地区：[1]西南农业大学蚕桑丝绸学院 [2]重庆市蚕业制种公司

出　　处：《蚕学通讯》1996年第2期12-16,共5页Newsletter of Sericultural Science

摘　　要：对700个母蛾用N.b碳素凝集反应法进行检微,碳检检出率为95.45%,对检的镜检检出率为22.72%.碳、镜检检出率的比值是4.2倍(即碳检有微数】镜检有微数);碳检发生的阳性反应样品,再用显微镜复检有微吻合率达100%,准确性大,误判率为零.检出微孢灵敏度高,最高N.b浓度是7.5×10~6个/ml,最低N.b浓度是2.5×10~3个/ml,可视为碳检700个母蛾批的检微浓度范围,以上数据证实用N.b致敏碳素检查母蛾微孢是可行的.N.b-carbon agglutination method (N.b-CAM) was employed to examine 700 female Moths, and the rate of female moths with N. b was 95.45% in the study, being 4.2 times ashigh as that of the control -the microscopic method (MM, 22. 72%). Samples of mothsshowing positive reaction in N. b-CAM were examined with MM and the rate of mothswith N. b was 100% ,the concentration ranging from 2. 5×103 to 7.5×106 spores/ml. It is,therefore, concluded that N. b-CAM is a reliable method for the examination of Nosemabombycis in female moths.

关 键 词：N.b碳素凝集反应 原种母蛾检微 

分 类 号：S884[农业科学—特种经济动物饲养]