沈阳地区部分献血员血清HBV—DNA检测及其意义  

Detection of Blood Serum HBV-DNA of Some Donors in Shenyang

作　　者：陈露秋[1] 马宝玺[1] 张菊娜 

机构地区：[1]沈阳医学院预防医学系流行病学教研室

出　　处：《沈阳医学院学报》1996年第1期43-44,48,共3页Journal of Shenyang Medical College

摘　　要：采用异羟基洋地黄毒苷配基标记HBV—DNA(Dig—HBVDNA)探针斑点杂交法检测了沈阳地区306例(男161,女145)献血员血清中HBV—DNA,并用ELISA法检测了HBeAg和抗—HBc。结果表明,血清HBV—DNA阳性者2例,阳性率为0.65％,与标准膜片色列比较估计HBV—DNA含量为1pg。血清HBeAg全部阴性,抗—HBc阳性者4例(其中1例HBV—DNA亦呈阳性)。说明经目前所采用方法筛检的献血员仍存在HBV感染者,有传播乙型肝炎的潜在危险性。306 'qualified donors' (male 161 and female 145, aged 20 to 50 years old) who were HB-sAg negative by ELISA were examined for blood serum HBV-DNA by Dit-HBVDNA probe spot hy-brization and for HBeAg, anti-HBc by ELISA in Shenyang. The results showed that the positive rate of HBV -DNA was 0. 65% (2/306) and it's content was about Ipg. All subjects were HBeAg negative and 4 anti-HBc were positive (one of them was HBV-DNA positive). So the present so-called qualified donors are undoubtedly playing a role of potential risk in transmission of post -transfusion hepatitis.

关 键 词：献血员 HBV-DNA 斑点杂交法 

分 类 号：R446.5[医药卫生—诊断学]