The influence of molecular structure of protein on the acoustic nonlinearity parameter B/A  

作　　者：LU Zhiqu GONG Xiufen WANG Shiquan LIU Jiehui and ZHANG Heyun(Institute ot Acoustics, State Key Lab. of Modern Acoustics, Nanjing University Nanjing 210093)(Department of Biochemistry Nanjing University Nanjing 210093) 

出　　处：《Chinese Journal of Acoustics》1996年第4期358-366,共9页声学学报（英文版）

摘　　要：In this paper, the influence of molecular structure of proteins on the acoustic non-linearity parameter B/A has been studied. By using biochemical technique, three proteins such as bovine serum albumin, bovine hemoglobin and pepsin were employed and their quarternary,tertiary and secondary protein structures were perturbed by the denaturing agent sodium do-decyl sulfate (SDS) solution, while their primary structures of protein were not changed. This means that only the structural features were altered and the chemical composition maintained unchanged. B/A has been measured by using improved thermodynamic method. The experi-mental results show that when the concentration of SDS solution increases, the alterations of the protein structure increase, and the contribution of protein to the B/A value decreases. Re-sults indicate that the B/A values can display the destruction of molecular structure of protein.Some explanation of these phenomena has also been made in this paper.In this paper, the influence of molecular structure of proteins on the acoustic non-linearity parameter B/A has been studied. By using biochemical technique, three proteins such as bovine serum albumin, bovine hemoglobin and pepsin were employed and their quarternary,tertiary and secondary protein structures were perturbed by the denaturing agent sodium do-decyl sulfate (SDS) solution, while their primary structures of protein were not changed. This means that only the structural features were altered and the chemical composition maintained unchanged. B/A has been measured by using improved thermodynamic method. The experi-mental results show that when the concentration of SDS solution increases, the alterations of the protein structure increase, and the contribution of protein to the B/A value decreases. Re-sults indicate that the B/A values can display the destruction of molecular structure of protein.Some explanation of these phenomena has also been made in this paper.

关 键 词：Acoustic nonlinearity parameter B/A Protein structure SDS solution 

分 类 号：Q62[生物学—生物物理学]