MALDI Mass Spectrometry Analysis of Human Hemoglobin  

作　　者：PENG Wei-xian(Department of Physics, Jilin University, Changchun, 130023) 

出　　处：《Chemical Research in Chinese Universities》1996年第1期44-49,共6页高等学校化学研究（英文版）

摘　　要：Matrix-assisted laser desorption/ionisation(MALDI) mass spectra in matrices DHBs and ferulic acid have been taken and used to determine the molecular weight of one of the variants of human hemoglobin. A single laser operating at 355 nm was used to irradiate the sample droplet spots on a target for both desorption and ionisation. Ions were analyzed and detected by a reflectron time-of-flight mass spectrometer system. The mass values of α-chain and β-chain without heme group for a kind of human hemoglobin molecules were determined, which are 15275 Da and 16060Da respectively, with the accuracy of 0. 03%. The various factors influencing the resolution of mass spectrum were discussed.Matrix-assisted laser desorption/ionisation(MALDI) mass spectra in matrices DHBs and ferulic acid have been taken and used to determine the molecular weight of one of the variants of human hemoglobin. A single laser operating at 355 nm was used to irradiate the sample droplet spots on a target for both desorption and ionisation. Ions were analyzed and detected by a reflectron time-of-flight mass spectrometer system. The mass values of α-chain and β-chain without heme group for a kind of human hemoglobin molecules were determined, which are 15275 Da and 16060Da respectively, with the accuracy of 0. 03%. The various factors influencing the resolution of mass spectrum were discussed.

关 键 词：LASER MATRIX HEMOGLOBIN 

分 类 号：O657.6[理学—分析化学]