AN　IMMUNOHISTOCHEMICAL　STUDY　OF　PERIVASCULAR　MACROPHAGES　IN　BRAINS　OF　SPONTANEOUSLY　HYPERTENSIVE　RATS  

作　　者：刘通 周敬德 房台生 凌凤东 

出　　处：《Journal of Pharmaceutical Analysis》1995年第2期130-132,138,共4页药物分析学报（英文版）

摘　　要：In order to investigate the possible role of macrophages in pathological changes of cerebrovascular diseases, we observed and counted the numbers of perivascular macrophages in brains of spontaneously hypertensive rats(SHR) and normotensive Wistar-Kyoto(WKY)rats using monoclon al antibodies(ED2)against rat macrophages.The immunohistochemistry(ABC method)and perfu sion-fixed frozen brain sections(16μm)were used in the study.The results showed that the ED2-specific staining in brain sections was restricted to macrophages in a perivascular location.The number of perivascular macrophages was significantly greater in SHR than in WKY.The results suggest that the hypertension is asseciated with increased subendothelial accumulation of macrophages.In order to investigate the possible role of macrophages in pathological changes of cerebrovascular diseases, we observed and counted the numbers of perivascular macrophages in brains of spontaneously hypertensive rats(SHR) and normotensive Wistar-Kyoto(WKY)rats using monoclon al antibodies(ED2)against rat macrophages.The immunohistochemistry(ABC method)and perfu sion-fixed frozen brain sections(16μm)were used in the study.The results showed that the ED2-specific staining in brain sections was restricted to macrophages in a perivascular location.The number of perivascular macrophages was significantly greater in SHR than in WKY.The results suggest that the hypertension is asseciated with increased subendothelial accumulation of macrophages.

关 键 词：ED2 macrophages:rat brain immunohistochemistry 

分 类 号：R174.2[医药卫生—妇幼卫生保健]