双抗体夹心酶联免疫吸附试验(S-ELISA)检测血吸虫病人循环抗原的研究  

DETECTION OF CIRCULATING ANTIGENS IN THE PATIENTS' SERA OF SCHISTOSOMIASIS JAPONICA BY DOUBLE-ANTIBODIES SANDWICH ELISA

作　　者：刘韵娟[1] 朱荫昌[1] 许永良[1] 何伟[1] 华万全[1] 管晓红[2] 仇镇宁[2] 

机构地区：[1]江苏省血吸虫病防治研究所,无锡214064 [2]南京医科大学寄生虫学教研室

出　　处：《中国血吸虫病防治杂志》1995年第6期345-348,共4页Chinese Journal of Schistosomiasis Control

基　　金：江苏省科技干部管理局部分经费资助

摘　　要：应用从重感染兔血清中提取的γ-球蛋白为捕捉抗体、以酶标单克隆抗体为结合物进行双抗体夹心酶联免疫吸附试验(S-ELISA),检测日本血吸虫病人循环抗原.结果:69例急性血吸虫病人血清的阳性率为80.6%一90.9%;110例慢性血吸虫病人血清的阳性率为88.2%;40例华支睾吸虫病人血清有1例出现阳性,交叉反应率为2.5%,50例健康人血清亦有1例出现阳性反应,假阳性率为2%.提示该法具有较高的敏感性、特异性,判断结果较客观,可应用于现场.In this paper the circulating antigens in the patients sera of schistosomiasis japonica were detected by Double-Antibodies Sandwich ELISA (S-ELISA),in which the γ-globulin extracted from the rabbits sera infected with 1 500 cercariae of Sj was used as the capture antibody,and the monoclonal antibody NP28-5B labeled horse radish peroxidase was used as the detecting antibody. The results showed that the positive rate in 69 cases of acute schistosomiasis was 80. 56%-90.91%;97 of 110 chronic schistosomiasis patients were positive,the positive rate was 88.18%), the cross reactive rate in 40 cases of clonorchiasis sinensis was 2. 5% (1/40), only one among 50 healthy pessons' sera was positive,the false positive rate was 2%. It suggestes that the method is senstive and specific,and determining reactive results objectively,there fore the method is applicable in the field examination.

关 键 词：日本血吸虫病 循环抗原检测 双抗体夹心ELISA 

分 类 号：R446.6[医药卫生—诊断学]