LACCASE－BASED　MICELLAR　ENHANCED　SPECTROFLUORIMETRIC　DETERMINATION　OF　L－ASCORBIC　ACID  

作　　者：Huang, HP Cai, RX Du, YM Zeng, YE 

机构地区：[1]Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China [2]Wuhan Univ, Dept Environm Sci, Wuhan 430072, Peoples R China

出　　处：《Chinese Chemical Letters》1995年第3期235-238,共4页中国化学快报（英文版）

摘　　要：An automated flow-injection stopped-flow micellar enhanced Spectrofluorimetric method for determination of L-ascorbic acid (AA) is described. The method is based on the interference of laccase by AA. causing a decrease in the rate of the laccase-catalysed fluorescence reaction in the presence of Brij-35. The resulting change of fluorescence intensity at em 530nm (ex430nm) is proportional to the concentration of AA with the linear range over 0. 2 to 2. 0μg/ml. The significant interference from ferric and cupric ions can be eliminated by addition of EDTA. The method has been applied to the determination of AA in pharmaceuticals. the results agree well with the nominal values and those obtained by the reference method. The recovery of AA in pharmaceuticals are between 91.5% and 115.4%.An automated flow-injection stopped-flow micellar enhanced Spectrofluorimetric method for determination of L-ascorbic acid (AA) is described. The method is based on the interference of laccase by AA. causing a decrease in the rate of the laccase-catalysed fluorescence reaction in the presence of Brij-35. The resulting change of fluorescence intensity at em 530nm (ex430nm) is proportional to the concentration of AA with the linear range over 0. 2 to 2. 0μg/ml. The significant interference from ferric and cupric ions can be eliminated by addition of EDTA. The method has been applied to the determination of AA in pharmaceuticals. the results agree well with the nominal values and those obtained by the reference method. The recovery of AA in pharmaceuticals are between 91.5% and 115.4%.

分 类 号：O62[理学—有机化学]