EFFECTS OF DIFFERENT COMBINATIONS OF CRYOPROTECTANTS ON SPERMATOZOON VITALITY OF CHINESE SCALLOP,CHLAMYS FARRERI,DURING LOW TEMPERATURE EQUILIBRIUM  

作　　者：薛钦昭 相建海 

出　　处：《Chinese Journal of Oceanology and Limnology》1995年第4期354-361,共8页中国海洋湖沼学报（英文版）

摘　　要：The effects of membrane penetrating anti-freezing agents(MPAAs), DMSO(dimethyl sulfoxide),glycerol,EG(ethylene glycol) and methanol in combination with different cryoprotective additives suchas carbohydrates,macromolecules and inorganic compounds on the spermatozoon vitality of Chinesescallop,Chlamys farreri,during 1 h 0℃ equilibrium were investigated.When only MPAAs existed,the detrimental effects of different MPAAs ranked in the following order:DMSO【methanol【EG【glycerol.When carbohydrates were added into MPAAs solution,5% glucose caused larger decrease ofspermatozoon vitality than 2.4% lactose.5% glucose or 2.4% lactose in 7.5% glycerol caused com-plete damage.10% yolk was best in maintaining the spermatozoon vitality except when used incombination with 10% methanol.10% milk significantly decreased spermatozoon vitality in EG andmethanol and enhanced its vitality in glycerol,but did not significantly influence it in DMSO.Glycinewas apparently detrimental to spermatozoon vitality.The averageThe effects of membrane penetrating anti-freezing agents(MPAAs), DMSO (dimethyl sulfoxide), glycerol, EG (ethylene glycol) and methanol in combination with different cryoprotective additives such as carbohydrates, macromolecules and inorganic compounds on the spermatozoon vitality of Chinese scallop, Chlamys farreri, during 1 h 0 'C equilibrium were investigated. When only MPAAs existed, the detrimental effects of different MPAAs ranked in the following order: DMSO<methanol<EG<glycerol. When carbohydrates were added into MPAAs solution, 5% glucose caused larger decrease of spermatozoon vitality than 2.4% lactose. 5% glucose or 2.4% lactose in 7.5% glycerol caused com-plete damage. 10% yolk was best in maintaining the spermatozoon vitality except when used in combination with 10% methanol. 10% milk significantly decreased spermatozoon vitality in EG and methanol and enhanced its vitality in glycerol, but did not significantly influence it in DMSO. Glycine was apparently detrimental to spermatozoon vitality. The average detrimental effects of additives in MPAAs ranked in the following order: 10% yolk< 10% yolk+ 2.4% lactose <2.4% lactose <2.0% citrate <10% milk < 10% milk +2.0% citrate <5% glucose < 80mmol/L glycine+55 mmol/L NaHCO_3.

关 键 词：SPERMATOZOON VITALITY CHLAMYS FARRERI CRYOPROTECTANTS EQUILIBRIUM 

分 类 号：Q492[生物学—生理学]