流行性出血热病毒感染NIH裸鼠免疫组织化学研究  

IMMUNOHISTOCHEMICAL STUDIES ON EPIDEMIC HEMORRHAGIC FEVER VIRUS INFECTION OF NIH NUDE MICE

作　　者：金朝杭[1,2] 毛庆波[1,2] 党锡连[1,2] 梁玉玲 陈现彬[1,2] 金艳 

机构地区：[1]济宁医学院流行性出血热研究室,济宁医学院免疫病理研究室 [2]济宁染料厂医院

出　　处：《中国组织化学与细胞化学杂志》1995年第3期283-285,共3页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：流行性出血热病毒（ＥｐｉｄｅｍｉｃＨｅｍｏｒｒｈａｇｉｃＦｅｖｅｒｖｉｒｕｓ，ＥＨＦＶ）感染ＮＩＨ裸鼠后，濒死状态取材，应用免疫组织化学方法（ＡＢＣ）检测各组织中的特异性病毒抗原。结果表明，ＮＩＨ裸鼠对ＥＨＦＶ感染敏感，感染后其脑、肺、肝、肾和心脏组织实质细胞浆内均可检出特异性抗原。The brain, lung, liver, spleen, heart and kidney tissues from NIH nude mice infected by epidemic hemorrhagic fever virus (EHFV) were detected by immunohistochemistry method (ABC) for EHFV antigen, on days. Result showed that the athymic NIH nude mice were highly susceptible to EHFV, and the infection could result in death of infected nude mice. The virus antigens could be regularly detected from various tissues and were found to be located in the cytoplasmas of cell of brain, lung, kidney, liver, spleen and heart.

关 键 词：流行性出血热病毒 抗原 免疫组织化学ABC 

分 类 号：R373[医药卫生—病原生物学]