胰岛素诱导低血糖大鼠心房肌细胞特殊颗粒的形态计量和心房利钠肽免疫组织化学研究  

STUDIES ON MORPHOMETRY OF SPECIFIC GRANULES AND IMMUNOHISTO CHEMISTRY OF ATRIAL NATRIURETIC PEPTIDE IN THE ATRIAL MYOCYTES OF INSULIN￣INDUCED HYPOGLYCEMIA RAT

作　　者：陈仙[1] 曾庆云[1] 丁成萦[1] 杨家齐[1] 

机构地区：[1]衡阳医学院附二医院外科教研室,湖北医科大学解剖学教研室

出　　处：《中国组织化学与细胞化学杂志》1995年第2期144-148,233,共6页Chinese Journal of Histochemistry and Cytochemistry

基　　金：湖北省教育委员会资助

摘　　要：通过形态计量学和免疫组织化学方法发现胰岛素诱导低血糖大鼠心房肌细胞核周区特殊颗粒（ＡＳＧ）的体密度、面数密度和数密度及平均直径均高于对照组（Ｐ＜０．０５），但高尔基复合体各参数与对照组比较没有差别（Ｐ＞０．０５）。实验组的心房利钠肽（ＡＮＰ）的免疫反应强度比对照组强（Ｐ＜０．００１）。提示胰岛素诱导低血糖对心房利钠肽的释放具有抑制作用，表明ＡＮＰ作为生理和病理调节递质与代谢刺激相拮抗。The morphometric and immunohistochemical methods were appliced to analyze the alteration of intracytoplamic composition(specific granules in the perinuclear region and GolgiComplexes)of the a trial myocytes from the insulin-induced hypoglycemia rats.The resultsshowed that there was a significant increase of volume density,numerical density on area,numerical denny and mean diameter of atrial specific granules(ASG) in the experimentalgroups(P<0.05),While the volume density and the numerical density on area of GolgiComplexes of the experimental groups were similar to the control rat's(P>0.05)and theimmunoreactive intensity of atrial natriuretic peptide(ANP)in the atrial myocytes of the experimental groups was much higher than that in the control groups (P<0.001).These findings suggest that insulin-induced hypoglycemia can inhibit the release of ANP.

关 键 词：心房利钠肽 心房特殊颗粒 胰岛素诱导低血糖 免疫组织化学 形态计量学 大鼠 

分 类 号：Q57[生物学—生物化学]