国人睾丸、附睾和输精管的转移酶组织化学观察  

ENZYME HISTOCHEMICAL OBSERVATION OF TRANSFERASES IN HUMAN TESTIS, EPIDIDYMIS AND VAS DEFERENS

作　　者：何才偆[1] 安劬[1] 

机构地区：[1]世界卫生组织人类生殖研究合作中心,四川省计划生育科学研究所

出　　处：《中国组织化学与细胞化学杂志》1995年第2期191-195,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：本文报告国人睾丸、附睾和输精管的ＡＣＴ，ＯＣＴ，ＨＧＫ，γ－ＧＴ，ＰＰＲ，ＢＥ，ＡＡＴ，ＣＫ和ＧＰＭ９种转移酶的酶组织化学定位及酶活性研究。结果显示：睾丸曲细精管的ＡＣＴ，ＨＧＫ和ＡＡＴ；睾丸间质细胞的ＨＧＫ和ＧＰＭ；睾丸输出小管和附睾管的ＡＣＴ，ＯＣＴ，ＨＧＫ，γ－ＧＴ，ＡＡＴ和ＣＫ；输精管的ＯＣＴ和ＧＰＭ的酶活性均呈强阳性或极强阳性。附睾头部的输出小管和附睾管含有极丰富的转移酶，这提示附睾头部功能尤其突出，为临床生育和不育提供重要指标．The testis,epididymides and vas deferens from 13 sudden unexpected death cases, aged19 - 38 years,were studied in this investigation with histochemical methods.The localization and activities of 9 kinds of transgerases in human testis,epididymis and vas deferenswere half quantitatively observed with light microscope.The results showed the activities offollowing enzymes were strong positive in following tissues and cells: ACT,HGK and AATin seminiferous tubles;HGK and GPM in Leydig cells;ACT,OCT,HGK,γ-GT,AAT andCK in ductuli efferente and ductus epididymis ; OCT and GPM in the vas deferens.The observations indicate that the ductuli efferentes and ductus epididymidis of the head of epididymis have plenty of transferases, and those enzymes may serve as the indexes to studysterility and fertility.

关 键 词：睾丸 附睾 输精管 转移酶 组织化学 人 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]