马桑内酯对培养的大鼠大脑皮层神经元γ─氨基丁酸、生长抑素分泌的影响  

EFFECT OF CORIARIA LACTONE ON γ-AMINOBUTYRIC ACID AND SOMATOSTATIN SECRETION OF CULTURED NEURONSOF RAT CEREBRAL CORTEX

作　　者：朱晓峰[1] 周洁萍[1] 孙桂荣 刘云华[1] 朱长庚[1] 

机构地区：[1]同济医科大学基础医学研究所脑研究室,佳木斯医学院

出　　处：《中国组织化学与细胞化学杂志》1995年第2期216-219,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金

摘　　要：本文利用培养的大鼠大脑皮层神经元，在不同浓度马桑内酯（５μｍｏｌ／Ｌ～２５０μｍｏｌ／Ｌ）作用后１２、２４、４８小时，应用双抗夹心法检测各时间点培养上清中γ－氨基丁酸和生长抑素含量。结果表明，各浓度中以２５μｍｏｌ／Ｌ马桑内酯作用最明显，在此浓度作用后１２小时，γ－氨基丁酸分泌受抑（抑制率为８．３％），生长抑素分泌则增加（增加率为２０．４％）。作用后２４、４８小时，γ－氨基丁酸分泌进一步受抑（抑制率为１０．６％、１４．５％），而生长抑素分泌则呈现增加幅度降低趋势（增加率为１１．７％、８．２％）。与未加药组相比，γ－氨基丁酸在三个时间点均有统计学意义（Ｐ＜０．０５），而生长抑素只在加药后１２小时有统计学意义（Ｐ＜０．０５）、本文对马桑内酯作用后两种神经递质变化的意义进行了讨论。The effect of coriaria lactone (CL)on γ -aminobutyric acid(GABA)and somatostatin(SOM)secretion were studied by tising cultured neurons of rat cerebral cortex.The range ofCL concentration was 5-250μmol/L.It was found that 25 μmol/L CL was suitable for thisstudy.GABA secretion was suppressed(the suppression rate was 8.3%),whereas SOM secretion was increased(the increase rate was 20.4%)after 12h. of using CL.GABA secretion was more evidently suppressed after 24hs and 48h. (Suppression rates were 10.6% and14.5%).Compared with the control group. GABA secretion were significantly suppressedat 12hs,24hs and 48hs.(P<0.05),but the significant increase of SOM was found only at12hs.The significance of GABA and SOM changes after using CL was disctlssed.

关 键 词：马桑内酯 大鼠 大脑皮质 γ-氨基丁酸 生长抑素 

分 类 号：Q42[生物学—神经生物学]