EFFECTS OF TURBULENT SHEAR STRESS ON THE GROWTH OF SUSPENDED PLANT CELLS IN A BUBBLE COLUMN REACTOR  

EFFECTS OF TURBULENT SHEAR STRESS ON THE GROWTH OF SUSPENDED PLANT CELLN IN A BUBBLE COLUMN REACTOR

作　　者：杨军政 胡宗定 王一平 

出　　处：《Chinese Journal of Chemical Engineering》1995年第2期42-49,共8页中国化学工程学报（英文版）

摘　　要：In this paper, effects of increasing the height of column, bubble size and gas flow rate on the growth of suspended Catharanthus roseus plant cells in a 1:5 litre bubble -column were studied. Statistical characteristics of turbulence such as auto-correlation function, and auto-power spectral density function of the fluctuating liquid velocity were measured in a bubble column employing pseudo-cell granulated agar. Calculations of turbulent shear stress were carried out. From these studies it is suggested that in plan! cell suspension culture sparged with large bubbles, turbulent shear stress is probably one of the most important reasons for the inhibited cell growth and cell damage. Larger bubbles or higher gas flow rates lead to the increase in shear stress and are more detrimental to plant cells.In this paper,effects of increasing the height of column,bubble size and gas flow rate onthe growth of suspended Catharanthus roseus plant cells in a 1.5 litre bubble column were studied.Statistical characteristics of turbulence such as auto-correlation function,and auto-power spectral den-sity function of the nuctuating liquid velocity were measured in a bubble column employing pseudo-cell-granulated agar.Calculations of turbulent shear stress were carried out.From these studies it is sug-gested that in plant cell suspension culture sparged with large bubbles.turbulent shear stress is prob-ably one of the most important reasons for the inhibited cell growth and cell damage.Larger bubblesor higher gas flow rates lead to the increase in shear stress and are more detrimental to plant cells.

关 键 词：CATHARANTHUS roseus SHEAR STRESS PLANT cell BUBBLE COLUMN 

分 类 号：Q691[生物学—生物物理学]