具有厌氧分解原油能力的一株BacillusD76  

A STRAIN OF BACILLUS D76 WITH THE CAPABILITY OF ANAEROBICAL BIODEGRADATION OF OIL

作　　者：刘春德[1] 江伯英[2] 郑莹[2] 王祖农[2] 王德民[3] 

机构地区：[1]大庆石油学院开发系 [2]山东大学 [3]大庆石油管理局

出　　处：《大庆石油学院学报》1993年第2期18-21,共4页Journal of Daqing Petroleum Institute

摘　　要：从大庆油田生产井中分离到一株BacillusDC76.该株菌为革兰氏阳性、能形成内生芽孢的菌株,具有还原销酸盐的能力,接触酶阳性。BacillusD76在以最终浓度为4%的大庆原油作为唯一碳原的无机盐场养基上,应用Hungate严格厌氧技术,40℃,150r/min培养25天,质谱分析检测到了丁酸和1,2,3,4-四甲基-5-异丙基苯的产生。该株菌在好氧条件下也能同化原油。A strain of Bacillus D76 was isolated from the producing well of Daqing Oilfield. Itis a Gram-positive, spore-forming aerobe. This strain reduces nitrates, and it is catalasepositive. On a reciprocal shaker (150 r/min) at 40℃, under strictly anaerobial conditionswhere Hungate technology was used, the strain of Bacillus D76 was grown on a mineralmedium containing Daqing oil as the carbon source in the final concentration of 4%. Bu-tanlic Acid and 1, 2, 3, 4-Tetra-Methyl-5-Isopropyl Benzene were detected by mass spec-trum. The strain of Bacillus D76 can also biodegradate oil aerobically.

关 键 词：原油 分解 厌氧微生物 

分 类 号：TE357.9[石油与天然气工程—油气田开发工程]