Evidence for the Formation of a New Variant Structure of λ-DNA in vitro  

作　　者：曹恩华 白春礼 何裕建 方晔 张平城 

机构地区：[1]Institute of Biophysics, Academia Sinica, Beijing 100101, PRC [2]Institute of Chemistry,Academia Sinica,Beijing 100080,PRC [3]Institute of Chemistry, Academia Sinica, Beijing 100080, PRC

出　　处：《Chinese Science Bulletin》1994年第3期208-211,共4页

基　　金：8.5 Project Grant of Academia Sinica.

摘　　要：In 1990, Bai et al.first directly observed an unusual triple-stranded braid-likeconformation of denatured λ-DNA Hind Ⅲ in vitro with scanning tunneling microscopy(STM). The triple-stranded braid-like conformation presented in this work was dif-ferent from the model of the right-handed triple helix DNA proposed by otherauthors.In the triple helix model, the third strand winds round the major groove ofthe double helix DNA. Several studies indicated that the triple-stranded DNA, whichwas prepared by the reaction of homopurine and homopyrimidine or single-strandedDNA and doube-stranded DNA, had a low melting temperatue (T_m), a highabsorbance at 280 run in the unwinding process and was resistant to DNase

关 键 词：Λ-DNA TRIPLE stranded DNA MELTING temperature. 

分 类 号：Q52[生物学—生物化学]