Effects　of　Phenamiphos　on　Muscarinic　Acetylcholine　Receptor　in　the　Brain　Synaptosome　in　Rats　and　Its　Mechanism  

作　　者：孙黎 马谭庚 刘毓谷 

出　　处：《Journal of Huazhong University of Science and Technology(Medical Sciences)》1994年第2期89-93,共5页华中科技大学学报（医学英德文版）

摘　　要：The studies were performed to investigate the mechanism of phenamiphos affecting muscarinic acetylcholine receptor (M-AchR) of the brain synaptosome in rats.The results showed that phenamiphos inhibited the binding of 3H-quinuclidinyl benzilate (3H-QNB) to the brain synaptosome in rats, and this effect was dose and time-depen dent. This suggests that phenamiphos might directly affect M-AchR. The KD and Bmax of phenamiphos to M-AchR were measured and found to be 1. 3 × 10-7 mol/L and 4. 57pmol/mg protein, respectively. It belongs to non-specific inhibition of phenamiphos on the binding of 3H-QNB to M-AchR. It was also found that phenamiphos decreased the level of protein sulfhydryl group in brain synaptosome membrane. Dithiothreitol (DTT) and the reduced glutathion (GSH) prevented the decrease of protein sulfhydrylgroup and the inhibition of phenamiphos on the binding of 3H-QNB to M-AchR. These showed that phenamiphos could affect the sulfhydryl group of M-AchR surface.The studies were performed to investigate the mechanism of phenamiphos affecting muscarinic acetylcholine receptor (M-AchR) of the brain synaptosome in rats.The results showed that phenamiphos inhibited the binding of 3H-quinuclidinyl benzilate (3H-QNB) to the brain synaptosome in rats, and this effect was dose and time-depen dent. This suggests that phenamiphos might directly affect M-AchR. The KD and Bmax of phenamiphos to M-AchR were measured and found to be 1. 3 × 10-7 mol/L and 4. 57pmol/mg protein, respectively. It belongs to non-specific inhibition of phenamiphos on the binding of 3H-QNB to M-AchR. It was also found that phenamiphos decreased the level of protein sulfhydryl group in brain synaptosome membrane. Dithiothreitol (DTT) and the reduced glutathion (GSH) prevented the decrease of protein sulfhydrylgroup and the inhibition of phenamiphos on the binding of 3H-QNB to M-AchR. These showed that phenamiphos could affect the sulfhydryl group of M-AchR surface.

关 键 词：organophosporous insecticides acetylcholine receptor sulfhydryl group 

分 类 号：R96[医药卫生—药理学]