葡萄糖与高脂血清对动脉平滑肌细胞结构的影响  

The effect of different concentrations of glucose with or without hyperlipidemic serum on the cytomorphology of arterial smooth muscle cells

作　　者：沈捷[1] 陈家伟[1] 刘铨之[1] 黄曼倩[1] 程宝庚[1] 薛婉芬[1] 周大虎[1] 

机构地区：[1]南京医科大学第一附属医院内分泌科

出　　处：《中国糖尿病杂志》1994年第3期139-143,共5页Chinese Journal of Diabetes

摘　　要：利用细胞培养技术，观察到：在伴与不伴高脂血清的，葡萄糖浓度为５．５５、１１．１、１６．７、３３．４以及５５．５ｍｍｏｌ／Ｌ各组别兔动脉平滑肌细胞之细胞形态学及细胞动力学改变，得到以下结果：①细胞蜕变与损伤程度和葡萄糖浓度呈正相关，与细胞增殖呈负相关（高脂和非高脂血清组相关度分别为ｒ＝－０．７９，Ｐ＜０．０１；ｒ＝－０．５９，Ｐ＜０．０１）。②高脂血清与葡萄糖之间对动脉粥样硬化的形成无协同作用。③在葡萄糖浓度大于１６．７ｍｍｏｌ／Ｌ组别细胞中，发现一种高电子密度样物质，系钙化小球，是动脉粥样硬化的成核部位。本文结果提示：保持糖尿病患者较低血糖血脂水平，可延缓动脉粥样硬化的发展。The observations on cytomorphology and cytodynamics of cultured rabbit arterial smooth muscle cells(SMC)under concentrations of glucose 5.55,11.1,16.7,33.4 and 55.5 mmol/L with or without hyperlipidemic serum(HLS)were carried out by light microscopy and transmission election microscopy. The results showed that①The extent of disruption in SMC was positively correlated with concentrations of glucose,and negatively correlated with the multipication ralte of DNA,a parameter of cytodynamics.②There was no synergistic effect between glucose and HLS in the development of atherosclerosis.③In high glucose concentration groups(glucose 16.7mmol/L)a calcifies particle of high electronic density like substance,probably the core of atherosclerosis was found in SMC. Our results suggest that to keep lower levels of blood glucose and blood lipids may delay the development of atherosclerosis.

关 键 词：葡萄糖 高脂血浆 平滑肌细胞结构 

分 类 号：R587.1[医药卫生—内分泌]