Further Studies on Structure of nodD3 Gene in Rhizobium meliloti——Analysis of 5'Non-Coding Region of nodD3 and Its Evolutionary Implications  

作　　者：俞冠翘 朱家璧 高云峰 沈善炯 

机构地区：[1]Laboratory of Molecular Genetics,Shanghai Institute of Plant Physiology,Academia Sinica,Shanghai 200032,PRC

出　　处：《Science China Chemistry》1994年第8期975-983,共9页中国科学（化学英文版）

基　　金：the National Natural Science Foundation of China.

摘　　要：R.meliloti nodD3 gene is transcriptionally controlled by two promoters.Gel retardationexperiments show that SyrM and NodD3 are binding to the first promoter region of nodD3,while no proteinfactor is found to bind to the second promoter region of the gene.Comparison has been made between 5′ non-coding region of nodD3 and the corresponding region upstream of nodD1.The presence of nod-box andnodA-like sequences in the 5′ noncoding region of nodD3 supports the hypothesis that nodD3 evolves withthe duplication of the nodD1-nodA fragment during the speciation of R.meliloti.R.meliloti nodD3 gene is transcriptionally controlled by two promoters.Gel retardation experiments show that SyrM and NodD3 are binding to the first promoter region of nodD3,while no protein factor is found to bind to the second promoter region of the gene.Comparison has been made between 5′ non- coding region of nodD3 and the corresponding region upstream of nodD1.The presence of nod-box and nodA-like sequences in the 5′ noncoding region of nodD3 supports the hypothesis that nodD3 evolves with the duplication of the nodD1-nodA fragment during the speciation of R.meliloti.

关 键 词：RHIZOBIUM MELILOTI nodD3 REGULATION EVOLUTION 

分 类 号：S154.3[农业科学—土壤学]