LABELING OF PROTEIN WITH A NEW EUROPIUM CHELATE OF 5-CHLOROSULFOYL-2-THENOYLTRIFLUOROACETONE(CTTA)AND APPLICATION TO IMMUNOASSAY  

作　　者：Yun Xiang CI Xiao Da YANG Chemistry Department,Peking University,Beijing 100871 

出　　处：《Chinese Chemical Letters》1992年第12期1007-1010,共4页中国化学快报（英文版）

基　　金：Supported by National Natural Science Foundation of China.

摘　　要：We describe for the first time the synthesis and the optimal conditions for protein labeling with a new fluorescent probe,5-chlorosulfoyl-2-thenoyltrifluoroacetone(CTTA),whicb forms a highly fluorescent conplex with Eu^(3+) when conjugated to protein.The labeled proteins were characterized by absorbance and fluorescence measurements and the effect of labeling on the biological activity of sone proteins was also studied.It is shown that the new label is suitable for applications in time-resolved fluoroimmunoassay.We describe for the first time the synthesis and the optimal conditions for protein labeling with a new fluorescent probe,5-chlorosulfoyl-2-thenoyltrifluoroacetone(CTTA),whicb forms a highly fluorescent conplex with Eu^(3+) when conjugated to protein.The labeled proteins were characterized by absorbance and fluorescence measurements and the effect of labeling on the biological activity of sone proteins was also studied.It is shown that the new label is suitable for applications in time-resolved fluoroimmunoassay.

关 键 词：Eu CTTA)AND APPLICATION TO IMMUNOASSAY LABELING OF PROTEIN WITH A NEW EUROPIUM CHELATE OF 5-CHLOROSULFOYL-2-THENOYLTRIFLUOROACETONE HSA 

分 类 号：O6[理学—化学]