烧伤侵袭性感染中三种常见细菌临界量的实验研究  

AN EXPERIMENTAL STUDY OF CRITICAL VALUE ON THREE COMMON BACTERIA INDUCING BURN WOUND INVASIVE INFECTION

作　　者：李利平 马恩庆[2] 

机构地区：[1]湖南医科大附一医院 [2]衡阳医学院附一医院

出　　处：《中国烧伤创疡杂志》1992年第1期29-32,66,共5页The Chinese Journal of Burns Wounds & Surface Ulcers

摘　　要：本文选择烧伤感染中三种觉见细菌即绿脓杆菌、金黄色葡萄球菌和大肠杆菌,分别建立大白鼠大面积Ⅲ度烧伤感染模型。伤后各组定期处死一定量的大白鼠行心血培养,创面组织病理学检查和组织匀浆细菌定量培养。结果表明绿脓杆菌毒力,侵袭力较强,易于血行扩散,金葡球菌和大肠杆菌毒力,侵袭力较弱,不易血行扩散;绿脓杆菌导致侵袭性感染的临界量为每克组织10~3个,金葡球菌为10~4,大肠杆菌为10~6。The Burn Wound invasive infection model was established in rats by comtamination of 15-20% body surface area full-thickness burn with pseudomonas aeruginosa, staphylococcus aureus, E coli, the most common bacteria in-ducing burn wound infection respectively. On preselected day, six or more in each group of rats were killed with pentobarbital sodium. Heart blood wass immediately aspirated for blood cul-ture. The burn wound examination including quantitative bacteriology culture and hist-opathology was performed in divided burn wound biopsy specimens. The results showed that pseudomonas aeruginosa was of more virulent and invasive and more easy blood dissemination, E coli, inversely, was of less virulent and invasive; Staphylococcus aureus and E coli were more diffi-cult blood dissemination; The critical values of pseudomonas aeruginosa, staphy- lococcus aureus, E coli respectively were 103、 104and 106bacteria/g tissue. It was the conclusion that different species of bacteria required different density to produce burn wound invasive infection. Key Words: Burnd Wound, Invasive Infec-tion. Blood culture, Histopathology, Bacterial Quantitation.

关 键 词：烧伤创面 侵袭性感染 血培养 组织病理学检查 细菌定量 

分 类 号：R82[医药卫生—临床医学]