小鼠桑椹期胚胎库的建立  

Establishment of the mouse morula embryo cryobank

作　　者：周吉盛[1] 时彦胜[1] 靳瞻群 汪宝珍[1] 

机构地区：[1]军事医学科学院实验动物中心,北京100071

出　　处：《制冷学报》1992年第3期37-41,共5页Journal of Refrigeration

基　　金：国家自然科学基金

摘　　要：在研究过程中,采用慢速和快速两种降温程序冻存小鼠桑椹期胚胎,两种方法都获得成功。从1987年8月至1991年底,共收集小鼠桑椹胚胎15900余枚,11个品种品系在液氮中进行冷冻保存。保存时间7个月至5年。取少量胚胎解冻后,24小时培养存活率是50——82.11％。冷冻胎解冻后移植产仔率达6.67——36.36％。对部分冷冻胎进行电镜观察和图象仪定量分析,证明其形态结构、图象定量分析与鲜胎无差别。对冷冻胎解冻后移植生出的小鼠进行遗传检测,证明其生化位点没有变化。对冷冻胎繁殖的后代进行有关生物试验,证明与原品系正常繁殖的小鼠实验结果一致。The first mouse morula embryos cryobank has been established in our laboratory. The frozen-thawed embryos were cultured, the survival rats were 50.00—82.11％. These frozen embryos in some inbred strains were transplanted, their born rats were 8.66—36.36％. These frozen and fresh embryos were observed and compared under a electron microscope and imagine analyzer for the first time. No matter whether they were frozen and fresh the difference between their morphological structure of the cells were insignificant.

关 键 词：小鼠桑棋期胚胎 冷冻保存 胚胎库 

分 类 号：TB6-55[一般工业技术—制冷工程]