DNA的apoB位点扩增片段长度多态性的检测及其在法医学中的应用  

AMPLIFICATION OF APOLIPOPROTEIN B 3'VNTR LOCUS BY PCR AND ITS APPLICATION TO FORENSIC SCIENCE PRACTICE

作　　者：李伯龄 倪锦堂 丁焰 陈松 胡兰 叶健 

机构地区：[1]公安部第二研究所,北京00038

出　　处：《中国法医学杂志》1992年第1期1-3,65,共4页Chinese Journal of Forensic Medicine

摘　　要：用PCR方法对100例无关个体血样的apoB位点扩增片段进行研究,已发现有11个等位基因,片段长度分布于600～1100bp之间,基因频率为0.005～0.525,杂合度为70％。家系分析及对人体血液、血斑、精液、精斑、混合斑、带毛囊毛发及其它各种有核细胞组织的研究表明,该技术在个人识别及亲子鉴定上均能发挥重要作用。The polymcrphisms of apolipoprotein B (apo B) VNTR locus were typed rapidly and accurately using polymerase chain reaction (PCR). Eleven alieles were identifed in 100 unrelated individuals with a heterozygosity of 70%. The allele frequencies were 0.5～52.5%. The size of amplified fragments were from 600 to 1100 bp. It has been demonstrated that DNA typing results can be obbained from biological materiais, such as senen stains, mixed stains,various tissues, blood stains, and single hair follicle.

关 键 词：apoB位点 PCR 扩增片段长度多态性(Amp-FLP) 可变数目串联重复(VNTR) 

分 类 号：D919[医药卫生—法医学]