Rowett大鼠眼内接种麻风菌的实验研究  

Intraocular Experimental Infection with M.Leprae in Nude Rats

作　　者：石珍荣[1] 周惠民[1] J.Kazda W.Mohr 

机构地区：[1]青岛医学院,青岛邮政编码266003 [2]德国Forschungsinstitut Borstel

出　　处：《中国麻风皮肤病杂志》1992年第2期75-78,共4页China Journal of Leprosy and Skin Diseases

摘　　要：以10只Rowett裸大鼠做眼内麻风菌接种。菌悬液的浓度为1.19×10~7条/毫升盐水(有6×10~5条活菌)。接种前超声振荡2分钟。在全身麻醉下,1～6号鼠用0.01～0.03毫升,7～10号鼠用0.01毫升,于右侧角膜外下方向前房接种;每只鼠均于左眼向玻璃体内接种o.03毫升.前者接种后有接种液由针眼溢出,其溢出量无法确定.接种后有1只死去,其余9只分别于第4～10周取其双眼,做石腊切片,用HE及Fire—Faraco染色.由于大鼠的晶体很大,有9只眼于按种时伤及晶体,引起晶体源性眼内炎,其中前房接种者6只,玻璃体接种者3只.在这些眼内晶体囊破裂,可见漏入玻璃体的晶体物质周围有致密的淋巴细胞和巨噬细胞浸润,间有巨细胞和中性白细胞;在浸润细胞周围有纤维组织形成,一直延伸到睫状体和视网膜;网膜有水肿、摺皱或萎缩;有些慢性炎性细胞侵及虹膜和睫状体.在9只晶体完整的眼内可见少量的淋巴细胞和巨噬细胞浸润,未见肉芽肿损害,其中3只为前房接种,6只为玻璃体接种。在所有的眼中均找到抗酸菌,几乎全为颗粒状。鉴于此,看来这种裸鼠不适于用来做眼内接种的眼麻风研究模型。Ten Rowett nude rats were inoculated intraoc- ularly with M. leprae suspension. The suspension was prepared with a concentration of 1. 19× 10~7 AFB/ml (with 6×10~6 living AFB) in normal saline, sonicated for 2 minutes before the inocula- tion. The inoculations were made afrer a general anesthesia of the animals. At the lateral inferior quadrate of cornea 0. 03 ml of the bacilli suspen- sion was injected into the anterior chamber of the right eyes of animals 1～6 and 0. 01 ml of the sus- pension into the anterior chamber of the right eyes of 7～10 animals. Into vitreous humor of the left eye of each animal 0. 03 ml of the suspension was injected. During the inoculations some leakage of the suspension was noticed but the amount of the leaked fluid was not able to be measured. Nine rats (one died just after the inoculation) were sacrificed at the 4th to 10th weeks after the inoculation and the eyes of the animals were enu- cleated and fixed, paraffin sections of the eyes were made and stained with hematoxylin and eosin and Fite--Faraco method. Due to trauma to the very large lens during in- oculation, phacogenic endophthalmitis occurred in nine eyes of the nine animals, of which six were inoculated in the anterior chambers and three in the vitreous humor. In these eyes the lens capsule was broken and around the lens material leaked into the vitreous body there were dense infiltration of lym- phocytes, macrophages, sometimes with giant cells, and neutrophiles. There were newly formed fibrous tissues around the infiltrating cells and the fibrous tissue was extended to the cilliary body and the retina. The retina was edematous, folding or a- trophic. Chronic inflammatory cells were infiltrat- ing the iris and cilliary body in various numbers. Of the nine eyes with intact lens, for three the bacill were inoculated into anterior chamber and for six into vitreous humor. In these eyes lymphcyte and small macrophage infiltration usually of mild degree was seen in the intraocular tissues. No gran- ulomatous lesion was

关 键 词：麻风菌 Rowett 大鼠 接种者 囊破 慢性炎性细胞 抗酸菌 中性白细胞 眼内炎 巨噬细胞浸润 

分 类 号：R75[医药卫生—皮肤病学与性病学]