应用PCR法检测患者尿中人巨细胞病毒DNA  

Detection of Human Cytomegalovirus in Human Urine by Polymerase Chain Reaction

作　　者：肖卫华[1] 董伟[1] 王玲[1] 徐德启[1] 汪革非[1] 

机构地区：[1]卫生部长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》1992年第3期121-123,共3页Chinese Journal of Biologicals

摘　　要：在人巨细胞病毒(HCMV)基因组早期蛋白基因处设计了一对引物,通过链聚合酶反应(polymerase chain reaction,PCR)扩增一段长106bp的特异性序列,用来检测样品中HCMV DNA。以此方法检测正常儿童尿标本阳性为15％(3/20),白血病患者尿阳性为76.2％(16/21),肾移植患者尿阳性为40％(13/33)。同时对部分标本进行了Dig标记的HCMV DNA探针法检测,结果PCR法检测的阳性率比Dig标记探针法高。We designed a pair of primer in the site of early protein gene of HCMV genome. It could be used to amplify a 106bp length sequence of HCMV to detect the HCMV Viral DNA in urine specimens. The viral DNA was amplified clireetly from 5μl of preheated urine. After 35 cycles of amplification, 50fg of a HCMV DNA which had no cross reaction with Herpse simplex virus type 1 or 2, 6, leukocyte cellular DNA, was detected. We tested the PCR assay on urine specimens from patients with renal transplant and leukemia, and found that there were 15% positivity in 20 normal children and 76.2% in 21 leukemia patients as well as 40% in renal transplant patients. We demonstrated that the PCR assay was more sensitive than the Dig-labeled probe.

关 键 词：人巨细胞病毒 聚合酶链反应 

分 类 号：R977[医药卫生—药品]