Vero细胞微载体培养乙型脑炎疫苗提纯方法的研究  

Study on the Purification of Japanese Encephalitis Virus Vaccine in Vero Cell-Microcarrier System

作　　者：陈志慧[1] 许一德 陈晓榕 宋家骊[1] 陈列胜[1] 陈文兰 李文勤[1] 陈燕[1] 

机构地区：[1]卫生部上海生物制品研究所,上海200052

出　　处：《中国生物制品学杂志》1992年第4期171-173,共3页Chinese Journal of Biologicals

摘　　要：首次应用国产20L Cellcul-20A型反应器和CT-2微载体Vero细胞培养乙型脑炎病毒。病毒液经福马林灭活后,澄清、浓缩、差速离心和除菌过滤,试制提纯乙型脑炎疫苗,抗原效价提高10倍以上,并建立提纯疫苗的检定方法。Japanese encephalitis virus which grew on Vero cells in microcarrier system was produced by using 20L Cellcul-20A bioreactor. The virus was inactivated by formalin, clarified, concentrated by hollow fiber ultra-filtration, and further purified by differential ultracentrifugation. The potency of the vaccine prepared by this method was increased more than 10-fold, and relevant control methods of the vaccine were established.

关 键 词：VERO细胞 微载体 乙型脑炎病毒 

分 类 号：R977[医药卫生—药品]