流行性乙型脑炎SA_(14)-14-2弱毒株在狗胎肾细胞传代适应的研究  

Study on Adaptation of Attenuated Japanses Encephalitis Virus Strain(SA_(14)-14-2PHK)to Primary Fetal Dog Kidney Cell Cultures

作　　者：王寿贵[1] 俞永新[2] 郑庆纹[1] 张大全 贾丽丽[2] 李玉华[1] 

机构地区：[1]卫生部成都生物制品研究所,成都610063 [2]中国药品生物制品检定所

出　　处：《中国生物制品学杂志》1992年第2期49-51,共3页Chinese Journal of Biologicals

摘　　要：流行性乙型脑炎SA_(14)-14-2弱毒株经原代狗胎肾细胞连续传递4代以后,其病毒滴度稳定在7.5～8.0 log TCID_(50)/0.2ml。传代后的各代病毒(命名为 SA_(14)-14-2PDK-CD)对三周龄小鼠脑内无毒力、皮下无致病力、弱毒力稳定性和免疫原性等特性与原 SA_(14)-14-2 株相同。以该株病毒和原代狗肾细胞制备的活疫苗,经全面检定完全符合现行《乙型脑炎活疫苗规程》标准。The Japanese encephalitis (JE) attenuated virus (SA_(14)-14-2pHK) was adapted to growin primary fetal dog kidney (PFDK) cell cultures.After four passages,the virus titer reached to 7.5log TCID_(50)/0.2ml.The adapted virus,SA_(14)-14-2PDK-CD,was avirulent and nonpathogenie to wean-ling mice by ic and sc inoculation and it also showed high progective effect tested in mice.The attenua-tion markers of SA_(14)-14-2PDK-CD remained stable after one back passage in suckling mouse brain,and all these characteristics were identical to those of SA_(14)-14-2PHK vaceine strain.The SA_(14)-14-2PDK-CD vaccine prepared in primary adult dog kidney cultures also met the mini-mum requirement for the production of Japanese encephalitis live vaccine for human use.

关 键 词：乙脑14-2弱病毒 原代狗胎肾细胞 病毒传代适应 乙脑活疫苗 

分 类 号：R977[医药卫生—药品]