人α_1型干扰素单克隆抗体在纯化人α_1型基因工程干扰素的应用  

A Report on The Monoclonal Antibody for the Purification of Recombinant Interferon α-1

作　　者：李财[1] 郭德本[1] 陈维佳[1] 陈云声[1] 刘景惠 

机构地区：[1]卫生部长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》1992年第1期9-11,共3页Chinese Journal of Biologicals

摘　　要：作者采用本所研制的人α₁型干扰素单克隆抗体,将人α₁型基因工程干扰素纯化至电泳纯级,平均得率为93％,平均比活性为6.5×10⁶ IU/mg蛋白,平均提纯倍数为15倍。3次试验经SDS-PAGF银染色法测定,均呈一条带,在非还原电泳条件下扫描干扰素纯度均在100％,残余鼠IgG含量测定均合乎规程要求,证明人α₁型单克隆抗体用于纯化人α₁型基因工程干扰素效果良好。Using the monoclonal antibody (McAb) to purify the human recombinant interferon (rHuIFN - α_1), could obtain an electrophoretic pure final product.The average recovery of rHuIFN was 93% and aveage specific activity was 6.5×10~6IU/mg protein. The purification factor was 15 fold in averge. Results of three tests, according to the methods recommended by National Institute for Control of Pharmaceutical and Biological Products, showed only single band by SDS-PAGE and 100% purity of the rHuIFN by staining under nonreducing condition. Analysed by ELISA, mouse IgG is less than 100ng per human dose of interferon. It has been proved that the McAb against rHuIFN is useful tool for purification of rHuIFN with satisfactory result.

关 键 词：人α₁型干扰素单克隆抗体 人α₁型基因工程干扰素 

分 类 号：R977[医药卫生—药品]