地高辛标记HBV DNA探针的应用  

Application of HBV DNA probe labelled with digoxigenin

作　　者：董伟[1] 肖卫华[1] 王玲[1] 贾国富[1] 翟立炜 于万龙[1] 徐德启[1] 

机构地区：[1]卫生部长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》1992年第1期19-22,共4页Chinese Journal of Biologicals

摘　　要：按随机引物法以地高辛标记HBV DNA探针,与按缺口翻译法以^(32)P标记的探针进行了比较。这两种探针与乙肝血源疫苗及患者血清标本中HBV DNA进行杂交,结果两种探针均可要出1pg同源序列DNA,而地高辛标记的探针,在0.1Pg水平仍可呈现弱着色反应。该探针在一20℃储存6个月,其敏感性不减弱。这种探针在常规诊断实验室更易推广使用。The whole HBV DNA sequences were labelled by random primer incorporation of Digoxigenin - labelled dUTP, and compared with ^(32)P-labelled probe by nick translation. The two probes were used to detect HBV DNA in plasma- derived HB Vaccine and sera of clinical patients by using Dot-blot hybridization. The results showed that the sensitivity of the two probes were comparable.Both of them could detect 1 pg of homologous DNA, but for the 0.1 pg level of HBV DNA could be detected only with a faint coloured precipitate by digoxigen-labelled probe. The stability experiments of the probe demonstrated that the sensitivity did not decreese after 6 months at -20℃. The non-isotope labelled probe is more readily applicable in routine diagnostic laboratories.

关 键 词：HBV DNA杂交 地高辛 

分 类 号：R977[医药卫生—药品]