细胞ELISA法的建立及应用  

The Development of Cell ELISA and Its Application

作　　者：田生和[1] 王大坤[1] 

机构地区：[1]卫生部武汉生物制品研究所,武昌430060

出　　处：《中国生物制品学杂志》1992年第1期27-29,共3页Chinese Journal of Biologicals

摘　　要：应用刀豆素蛋白A为结合剂将细胞结合于酶标板的细胞ELISA法(C-ELISA),检测几种抗细胞膜抗原单抗和筛选抗细胞膜抗原单抗,均显示具有较好的特异性和重复性。在筛选杂交瘤上清时,该法较间接免疫荧光法敏感、简便。A cell ELISA (C - ELISA) was developed in which concanavalin A was used as a binding agent between microtiter plate and cells.This method showed a good specificity and stability in detecting and screening monoclonal antibodies against cell surface antigens and was more sensitive and simpler than the indirect membrance immunofluorescence.

关 键 词：刀豆素蛋白A 细胞ELISA 单克隆抗体 

分 类 号：R977[医药卫生—药品]