PAF拮抗剂抑制环孢素A诱导的肾系膜细胞收缩作用的研究  

The inhibitory effect of PAF antagonist-BN52021 on CsA induced mesangial cell contraction

作　　者：高勇[1] 张本立[1] 梅长林[1] 

机构地区：[1]上海第二军医大学长征医院肾脏内科,200003

出　　处：《肾脏病与透析肾移植杂志》1992年第1期15-18,共4页Chinese Journal of Nephrology,Dialysis & Transplantation

摘　　要：本文采用SD大鼠肾脏系膜细胞培养及硅胶膜形态分析技术。研究环孢素A(CsA)诱导肾系膜细胞收缩作用及血小板活化因子拮抗剂BN52021对CsA诱导作用的抑制效应。结果示CsA引起系膜细胞收缩呈现时间和剂量依赖效应,其作用可逆。BN52021浓度为5×10^(-5)M时,可显著地抑制CsA的诱导作用。结果提示CsA通过可逆性诱导系膜细胞收缩引起肾小球毛细血管血流量和超滤系数(Kf)下降;血小板活化因子可能参与了这个过程,使用血小板活化因子拮抗剂可能有预防及治疗CsA肾毒性的作用。In this paper, the method of cultured rat mesangial ceils (Ms ceils) and morphologic analysis of Ms cells with DMPS membrane were firstly established. The effect of Cyclosporine A (CsA)on the Ms cells contraction and the protection of Ms cells from CsA toxicity using platelet-activating factor (PAF) antagonist-BN52021 were observed. In results, CsA induced Ms cells contraction in time and concentration dependent manner. CsA (10^(-9)M) also had the effect and lasted half an hour. Twenty minutes after removing of CsA from cells, the Ms cells could recover from their contracting states. Ms cells contraction induced by CsA was significantly inhibited by PN52021, at 5×10^(-5)M. The results indicate: 1) The effect of CsA induced Ms cells contraction is reversible and the contraction of Ms cells can reduce renal blood ilow and glomerular capillary kf in rive. 2) PAF may take part in the cell contraction in some way. PAF antagonist BN52021 has the effect on prophylaxis and treatment of CsA nephrotoxicity.

关 键 词：环孢素A 系膜细胞 BN52021 

分 类 号：R5[医药卫生—内科学]