Actional Mechanism of Trifluoroacetic Acid for the Separation of Biopolymers by Reversed-phase Liquid Chromatography  

作　　者：SHI Ya-li and GENG Xin-du (Department of Chemistry, Northwest University, Xi’an, 710069) 

出　　处：《Chemical Research in Chinese Universities》1992年第3期207-211,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natrual Science Foundation of China

摘　　要：The present paper covers the actional mechanism of trifluoroacetic acid for the separation of biopolymers investigated by using the parameters of stoichiometric displacement model for retention(SDM-R) in reversed-phase liquid chromatography. It was found that the trifluoroacetic acid(TFA) may participate in, or stimulate the association among displacing agent molecules in mobile phase, and decrease the affinity of both the associate molecules of the displacing agent and the TFA-protein ion-pairing. The former dominates over the separation selectivity of biopolymers as the concentration of TFA is lower than a given value, and the two contrary functions partly offset to each other and the latter dominates as its concentration is greater than the given value.The present paper covers the actional mechanism of trifluoroacetic acid for the separation of biopolymers investigated by using the parameters of stoichiometric displacement model for retention(SDM-R) in reversed-phase liquid chromatography. It was found that the trifluoroacetic acid(TFA) may participate in, or stimulate the association among displacing agent molecules in mobile phase, and decrease the affinity of both the associate molecules of the displacing agent and the TFA-protein ion-pairing. The former dominates over the separation selectivity of biopolymers as the concentration of TFA is lower than a given value, and the two contrary functions partly offset to each other and the latter dominates as its concentration is greater than the given value.

关 键 词：Trifluoroacetic acid Actional mechanism Reversed-phase liquid chroma-tography SEPARATION Biopolymers 

分 类 号：O6[理学—化学]