大鼠实验性脾虚证胃粘膜内分泌细胞变化的免疫组织化学研究  

IMMUNOHISTOCHEMICAL STUDY ON THE ENTEROENDOCRINE CELLS OF GASTRIC MUCOSA IN "EXPERIMENTAL SPLEEN DEFICIENCY SYNDROME" IN THE RATS

作　　者：王秀琴[1] 杨进[1] 刘绍杰[1] 刘英琴[2] 李玲风 韩秀兰[1] 王玉慧[2] 

机构地区：[1]首都医学院组织胚胎学教研室 [2]首都医学院生物工程系数学教研室,北京100054

出　　处：《中国组织化学与细胞化学杂志》1992年第1期49-56,138,共9页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：用正常成年雄性Wistar大鼠53只,体重100～150g,分为正常对照组、实验性脾虚组、自然恢复组和四君子汤治疗组。取胃,固定于Bouin液。制成石蜡切片,进行(1)HE染色;(2)免疫组织化学染色,按Sternberger PAP法显示胃泌素细胞(G细胞)、生长抑素细胞(D细胞)和5-HT细胞。根据细胞的免疫反应程度,将细胞分为强阳性、中等阳性和弱阳性三级,每例动物计数三种细胞各1,000个,并计算各级细胞占的百分比;(3)从正常对照组,脾虚组随机选择各5例动物,对D细胞和G细胞进行显微分光光度计的定量测定;(4)由四组动物随机选择各5例,进行G细胞和D细胞密度及G/D细胞比值的测定。本文的观察表明:(1)脾虚组胃粘膜未见明显的组织学变化;(2)内分泌细胞:与对照组相比,脾虚组G细胞和5-HT细胞中,弱阳性细胞增多,表明分泌活动增强;D细胞弱阳性和中等阳性细胞减少,强阳性细胞增多,表明分泌释放减弱,合成增强。与自然恢复组比较,治疗组G细胞和D细胞的分泌活动接近于对照组;5-HT细胞无明显的恢复;(3)显微分光光度计的测定结果与光镜观察一致,脾虚组G细胞胃泌素反应物的含量低于对照组,D细胞内生长抑素反应物的含量高于对照组;(4)脾虚组G细胞密度低于对照组(P<0.01),D细胞密度略高于对照组,G/D细胞比值也低于对照组(P<0.01)。本文结果提示,脾虚证这些内分泌细胞的分泌活动出现异常,可能是导致消化功能紊乱的原因之一。经四君子汤治疗后,内分泌细胞的分泌活动接近于对照组,说明此药对脾虚证有一定的治疗作用。53 adult Wistar male rats were used and divided into 4 groups, i.e. normal control group, 'experimental spleen deficiency group' induced by rhubarb, spontaneous recovery group and therapeutic group treated with Chinese herbs (Si Jun Zi decoction). All the animals of the 4 groups were killed and processed for demonstration of gastrin cells (G cells), somatostatin cells (D cells) and 5-HT cells according to immunohistochemical PAP technique. H-E stained-preparations were prepared for observation of histological changes of gastric mucosa. The immunoreactivities of the three types of enteroendocrine cells were observed and semiquantitative estimation was made with light microscopy. The number of strong positive (+++), intermediate positive (++) and weak positive (+) cells was counted and the percentage of positive cells of each grade was calculated. In addition, the immunoreactivities of G cells and D cells in normal control group and 'experimental spleen deficiency' group were measured by microspectrocytophotometer (MPV-2, Leitz). The density of G cells and D cells (cell/mm^2 mucosa) was measured by image analysor (pericolor 2001, France) and G/D cell ratio was calculated. All the data were analyzed statistically. This study revealed that there were no obvious histological changes in the gastric mucosa in all the 4 groups. The percentage of G cells(+), 5-HT cells(+), D cells(+++) in the 'experimental spleen deficiency group' was higher than that of the normal control group, while the percentage of G cells(+++), 5-HT cells(++), D cells (+,++) was lower than that of the normal control one. The obvious difference between these two groups was showed. (P<0.01) As compared with spontaneous recovery group, the percentage of G cells (+,++,+++), 5-HT cells (++) and D cells (+,++,+++) in the therapeutic group was approximated to the normal control group. The immunoreaetivity of G cells and D cells measured with microspectrophotometer appeared consistent with the semiquantitative results. The density of C cells and G/D cell ra

关 键 词：实验性脾虚证 免疫组织化学 胃泌素细胞 5-HT细胞 生长抑素细胞 大鼠 

分 类 号：Q[生物学]