流式细胞光度术DNA分析在非何杰金氏淋巴瘤预后中的意义  

PROGNOSTIC SIGNIFICANCE OF DNA FLOW CYTOMETRY IN NON-HODGKIN'S LYMPHOMA

作　　者：吴焕明[1] 

机构地区：[1]同济医科大学病理学教研室,武汉430030

出　　处：《中国组织化学与细胞化学杂志》1992年第1期60-64,共5页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：用流式细胞光度术对一组非何杰金氏淋巴瘤(NHL)存档石蜡标本进行DNA分析。DNA异倍体肿瘤占总例数的47.4％,高、中度恶性组异倍体肿瘤的比例(76.8％和51.9％)分别高于低度恶性组(17.6％)(P<0.05)。S期细胞比例(synthetic phase fraction,SPF)随组织学恶性程度升高而增大,低、中和高度恶性淋巴瘤平均SPF值分别是6.5(0.6—18.8)％,13.5(3.2—37.3)％和23.4(4.4—41.4)％。二倍体和异倍体肿瘤患者的生存率无差别。全部病例中高SPF值肿瘤患者具有生存短的趋向(P=0.1)。低度恶性组内也存在此种趋向(P=0.08)。上述结果表明流式细胞光度术DNA分析对估价非何杰金氏淋巴瘤生物学行为是一种不依赖形态学的有用方法。A series of cases of non-Hodgkin's lymphoma were studied by flow cytomerry. The DNA analysis was performed by applying a trypsin digestion method to archival paraffin-embedded samples. DNA aneuploid tumors constituted 47.7％ of the total, and the frequency in the high and intermediate grade group (76.8％ and 51.9％) was markedly higher than that in the low grade group (17.6％) (P<0.05). The S phase fraction (SPF) increased significantly with the high histological malignant grade (P<0.005). The mean SPF value in the low, intermediate and high grade lymphomas was 6.5 (0.6-18.8)％, 13.5(3.2-37.3)％ and 23.4 (4.4-41.4)％ respectively. The survival rate of patients with diploid and aneuploid tumor was not different. There was a trend of shorter survival rate for cases with high SPF value in all cases and low grade group of lymphomas (P=0.1 and P=0.08 respectively). The results show that the flow cytometric analysis of DNA content for prediction of prognosis in non-Hodgkin's lymphoma should be a useful method of independent morphology.

关 键 词：非何杰金氏淋巴瘤 流式细胞光度术 DNA分析 

分 类 号：Q[生物学]