三种绦虫蛋白质等电聚焦电泳比较分析  

COMPARATIVE ELECTROPHORETIC PROTEIN ANALYSIS OF THREE SPECIES OF CESTODES BY ISOELECTRIC FOCUSING

作　　者：佟小莺[1] 白功懋[1] 刘明洲[2] 林红[2] 

机构地区：[1]天津第二医学院寄生虫学和基础生化 [2]天津第二医学院教研室

出　　处：《畜牧兽医学报》1992年第4期336-340,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：应用PAGE-IEF分析了三种绦虫的可溶性蛋白质。猪囊尾蚴头节囊壁、囊液,细颈囊尾蚴头节囊壁、囊液和曼氏迭宫绦虫成虫蛋白质区带数分别为30、25、29、18和24～29条,主带(峰)数分别为10、9、9、7和12个,面积最大峰分别为J、B、H、F和E峰。五种样品电泳谱在总带数、各pH区带数、主带(峰)数和分布、面积最大峰都明显不同,可供鉴定和鉴别。细颈囊尾蚴蛋白质电泳分析与猪囊尾蚴的比较分析,以及曼氏迭宫绦虫蛋白质电泳分析系国内外首次报道。Soluble proteins of three species of cestodes were analyzed electro-phoretically by isoelectric focusing in polyacrylamide gels, The numbers of protein bands isolated from the scolex-cyst wall of Cysticercus cellu-losae, cystic fluid of C, cellulosae, scolex-cyst wall of C. tenuicollis, cystic fluid of C. tenuicollis and adult Spirometra mansoni were 30, 25, 29, 18 and 24-29 respectively, the numbers of prominent bands ( peaks) were 10, 9 , 9 , 7 and 12 respectively, and the peaks with biggest area were J, B, H, F and E respectively. The electrophoretic profiles of the five samples were significantly different in the total number of bands, number of bands in different pH regions, number and distribution of prominent bands ( peaks ) as well as peaks with biggest area, being useful in identification and differentiation. The study is the first report on the electrophoretic protein analysis of C. tenuicollis and its comparison with C. cellulosae, and that of Spirometra mansoni.

关 键 词：蛋白质 电泳分析 等电聚焦 猪囊尾蚴 细颈囊尾蚴 曼氏迭宫绦虫 

分 类 号：S8[农业科学—畜牧兽医]