A NEW SPIROPLASMA FROM THE SURFACE OF PLANT FLOWER IN CHINA  

作　　者：郭永红 叶旭东 陈永萱 CHEN T.A. 

机构地区：[1]Departmant of Plant Protection, Nanjing Agricultural University [2]Department of Plant Pathology, Rutgers University

出　　处：《Science China Chemistry》1991年第3期290-296,共7页中国科学（化学英文版）

基　　金：Project supported by the National Natural Science Foundation of China. Part of this research was done in Rutgers University, U.S.A.

摘　　要：The strain CCH, isolated from the surface of the plant flower in China, is a wall- less,helical prokaryote which passes through a microfilter 0. 22 μm in diameter. Cholesterol isrequired for its growth. The strain CCH could metabolize glucose, fructose, maltose, treha-lose and arginine, but not urea. Its electrophoresis pattern of cell proteins is distinct fromthat of other spiroplasmas. The guanine-plus-cyrosine content of its DNA determined bymelting temperature is 29. 15 mol%. No relation with any known spiroplasmas was found inenzyme-linked immunosorbent assay and deformation tests. According to these results, thestrain CCH is referred to a new species in the genus of Spiroplasma or the representative ofa new spiroplasma group.The strain CCH, isolated from the surface of the plant flower in China, is a wall- less, helical prokaryote which passes through a microfilter 0. 22 μm in diameter. Cholesterol is required for its growth. The strain CCH could metabolize glucose, fructose, maltose, treha- lose and arginine, but not urea. Its electrophoresis pattern of cell proteins is distinct from that of other spiroplasmas. The guanine-plus-cyrosine content of its DNA determined by melting temperature is 29. 15 mol%. No relation with any known spiroplasmas was found in enzyme-linked immunosorbent assay and deformation tests. According to these results, the strain CCH is referred to a new species in the genus of Spiroplasma or the representative of a new spiroplasma group.

关 键 词：NEW FLOWER SPIROPLASMA MORPHOLOGY and characterization identification 

分 类 号：O6[理学—化学]