非同位素标记DNA探针初步研究  

THE APPLICATION OF NONISOTOPIC DNA PROBE IN FORENSIC SCIENCE

作　　者：倪锦堂 叶健 刘健 郑秀芬 李伯龄 丁焰 

机构地区：[1]公安部第二研究所,北京100038

出　　处：《中国法医学杂志》1991年第3期129-131,194,共4页Chinese Journal of Forensic Medicine

摘　　要：本文报导了将辣根过氧化物酶直接标记在单链 DNA 探针上,制备出非同位素的 DNA 探针,采用化学光增强法检测杂交结果,所得图谱清晰,容易判读。所制备出的 DNA 探针可用于人类性别鉴定和个体识别,为 DNA 检验技术推广应用开辟了新的领域。A method of labelling nucleic ac id probes with nonisotopic reagents is described.DNA probes were directly labelled with horseradish peroxidase(HRP).The clear a-nd readable patterns were obtained by the enhanced chemiluminescence.The noni-sotopic DNA probes were widely used in both sexual and individual identificati-ion.

关 键 词：DNA 探针 个体识别 非同位素 辣根过氧化物酶 

分 类 号：D919[医药卫生—法医学]