鼠抗角蛋白单克隆抗体的研制及在肿瘤分类诊断中的应用  

Preparation and Characterization of Monoclonal Anti-keratin Antibodies and use in Tumor Classification

作　　者：谷正贤 张碧如[1] 史秀云 韩璞 程明[1] 

机构地区：[1]卫生部北京生物制品研究所,北京100024

出　　处：《中国生物制品学杂志》1991年第2期60-65,共6页Chinese Journal of Biologicals

摘　　要：以人皮肤、牛鼻皮肤及 Hela 细胞角蛋白作免疫原,应用鼠——鼠杂交瘤技术获得了三株在肿瘤分类诊断中具有应用价值的抗角蛋白单克隆抗体。其中 HK_2和 K_(174)既可识别大分子角蛋白,又与小分子角蛋白反应,因而在免疫组化染色中对复层上皮、单层上皮及鳞癌和腺癌细胞都呈阳性反应。但 HK_2对起源于单层上皮、腺上皮癌的反应优于 K_(174),而 K_(174)则对鳞癌的反应比 HK_2强。二者的混合单抗对低分化和未分化癌的免疫组化染色阳性率达87.1%。K_(27)则主要识别大分子角蛋白,故仅与复层上皮基底层以上细胞及鳞癌反应,而单层上皮及肺部肿瘤以外的腺癌呈阴性反应,故可用于除肺部肿瘤以外的鳞癌和腺癌的分类诊断。Three monoclonal anti-keratin antibodies which can be used in tumor cl-assification have been obtained.HK_2 and K_(174) can recognize both higher and lowermolecular weight keratins,and reacts not only with squamous epithelial cells and sq-uamous cell carcinomas,but also with simple epithelial cells and adenocarcinomas byimmunohistochemical staining.However,HK_2 showed better reaction with carcinomasoriginated from simple and gland epithelial cells than K_(174).K_(174) reacted more stronglywith squamous cell carcinomas than HK_2.The positive rate of low-differentiated andundifferentiated carcinomas stained with mixed HK_2 and K_(174) was 87.1%.K_(27) mainly re-cognized higher molecular weight keratins,and thus only reacted with superbasal la-yers of squamous epithelial and squamous cell carcinomas.However,the basal layer ofsquamous epithelium,simple epithelium and adenocarcinomas (with exception of lungadenocarcinomas) were all negatively stained with K_(27).Thus,K_(27) is useful for diffe-rentiating squamous cell carcinomas and adenocarcinoma,except lung tumor.

关 键 词：角蛋白 上皮细胞 免疫组化 肿瘤分类 

分 类 号：R977[医药卫生—药品]