一株有模拟CEA表位作用的抗独特型抗体  

Anti-CEA Response Induced by Anti-idiotypic Antibody

作　　者：全昱东 蒋先敏[1] 赵莉[1] 程明[1] 

机构地区：[1]卫生部北京生物制品研究所,北京100024

出　　处：《中国生物制品学杂志》1991年第3期105-108,共4页Chinese Journal of Biologicals

摘　　要：为了获得 CEA(癌胚抗原)表位内影象型抗体(抗独特型抗体 Ab_2 β),我们选用同系小鼠作为免疫动物,从而避免了用异种动物制备 Ab_2 时碰到的抗同种抗体和抗同种异型抗体对分析工作的干扰。抗 CEA 单克隆抗体(MAb)C50免疫同系 BALB/c 小鼠后,应用杂交瘤技术获得了2株 MAb,对其中一株 ID56进一步鉴定,发现 ID56仅与 C50有结合作用。不与另外2株抗 CEAMAb C6和 C14结合,ID56还能抑制 C50与 CEA 的结合反应,其腹水的竞争抑制效价为1∶64左右。ID56免疫同系小鼠产生了抗血清Ab_3 与 CEA 结合效价达1∶160,从而表明 MAb 56是 C50所识别的表位内影象。Anti-idiotypic antibody preparation is a novel way to develop vaccines,especially in the hope of modulating the immune response of tumor patients.We used monoclonal anti-carcinoembryonicantigen antibody C50 to make monoclonal anti-idiotypic antibodies from syngeneic mice.BALB/C micewere immunized with antibody C50 conjugated to KLH.By means of hybridoma technique,two monocloneswere obtained.One of them ID50 was further characterized.It could bind to Ab C50,but not to the othertwo anti-CEA antibodies C6 and C14,Which recognize different epitopes from Ab C50.Furthermore,AbID56 could inhibit Ab CS0to bind with CEA.Anti-ID56 sera prepared from syngeneic mice could reactwith CEA.The result strongly indicated that Ab ID50 is a Ab2 anti-idiotypic antibody i.e.internalimage,which can mimic CEA epitope to induce response in vitro.

关 键 词：独特型 抗独特型抗体 MAB 抗原内影象 CEA 

分 类 号：R977[医药卫生—药品]