抗急性非淋巴细胞白血病M_5型单克隆抗体研究  

Research on M_5-type Monoclonal Antibody Against Acute non-Lymphocytic Leukemia

作　　者：张权一[1] 潘荆华[1] 李秀芝[1] 张颖超 吴铁南 王诚[1] 王晓薇[2] 李长平 易永林[2] 

机构地区：[1]卫生部长春生物制品研究所,长春130062 [2]白求恩医科大学第一临床学院,长春130021

出　　处：《中国生物制品学杂志》1991年第4期154-156,共3页Chinese Journal of Biologicals

摘　　要：用急性非淋巴细胞白血病(急非淋)M_5型白血病相关抗原免疫 BALB/C 小鼠,取免疫鼠脾细胞与 NS-1骨髓瘤细胞融合,建立了2株能稳定分泌抗急非淋 M_5型白血病抗体的杂交瘤株。所分泌的单克隆抗体与急非淋 M_5型白血病有反应,与其他型白血病没有交叉反应,具有很好的特异性。CFU-GM 培养结果对骨髓造血干细胞没有影响。BALB/C mice were immunized with the M_5-type associated antigen of acute non-lymphocytic leukemia,The spleen cells were fused with NS-1/1.Aq.4.1 m yeloma cells, and 2 cell strains which could secret anti-acute non-lymphocytic leukemia M_5-type monocl- onal antibodies were established.All the monoclonal antibodies secreted by these cell stra- ins gave positive reaction with the leukemia cells from acute non-lymphocytic leukemia M_2- type patient,but could not give any reaction with the cells from other type of leukemia patients.Themonoclonal antibodies produced by the cell strains showed good specificity.The result of CFu-GM culture did not affect myeloid stem cells.

关 键 词：急性非淋巴细胞白血病 杂交瘤 单克隆抗体 

分 类 号：R977[医药卫生—药品]