治疗用抗人T淋巴细胞分化抗原单克隆抗体与人体组织反应性的体外研究  

The Reactivities of Anti-Human T Lymphocyte Differe ntiation Antigen MAbs for Therapeutical Use with Normal Human Tissues In Vitro

作　　者：谭向阳[1] 陈善华[1] 史良如[1] 

机构地区：[1]卫生部武汉生物制品研究所,武昌430060

出　　处：《中国生物制品学杂志》1991年第4期157-160,共4页Chinese Journal of Biologicals

摘　　要：应用免疫组化酶染色法及免疫细胞荧光染色法体外检测了 WuT_3(抗 CD_3),WuT_4(抗CD_4),WuT_8(抗 CD_8),WuT_9(抗 CD_71 或 TfR),WuTac(抗 CD_(25)或 IL-2R)等5种临床治疗用小鼠抗人 T 淋巴细胞分化抗原单克隆抗体(简称单抗)与25种正常人体组织的反应性。结果显示,WuT_3与髓质胸腺细胞,淋巴结及扁桃体中 T 细胞区细胞,脾白髓中动脉周围淋巴鞘细胞及胃肠道粘膜层淋巴细胞呈阳性反应。WuT_4与皮质胸细胞及大多数髓质胸腺细胞呈阳性反应。WuT_8与皮质胸腺细胞及少数髓质胸腺细胞呈阳性反应。WuT_4 和 WuT_8阳性细胞在淋巴结、扁桃体、脾脏及胃肠道粘膜中的分布与 WuT_3 阳性细胞基本一致,但数量依次减少。淋巴结和扁桃体的生发中心以及脾脏的脾小结中散在分布有少数WuT_3,WuT4及 WuT_8阳性细胞。此外,睾丸中散在分布个别 WuT_8阳性淋巴细胞。WuT_9与26%的骨髓细胞及个别皮质胸腺细胞呈阳性反应。WuT_9 尚与小肠粘膜肠腺底部细胞呈较强染色。WuTac 仅与个别散在的髓质胸腺细胞呈阳性反应。上述5种单抗与其它非淋巴组织及细胞基本呈阴性反应。By using the techniques of immunohistological enzyme staining and immunoc- ytological fluorescent staining,the reactivities of WuT 3(anti-CD3),WuT4(anti-CD4), WuT8(anti-CD8),WuT9(anti-CD71 or TfR)and WuTac(anti-CD25 or IL-2R)MAbs with 25 normal human tissues in vitro were detected.The results showed that medullary th- ymocytes,the T cell area cells in lymph nods e and tonsil,the arterial lymphatic sheath cells in spl- een and the scattered Iymphocytes and lymph nodule cells in gastrointestinal mucosa were stain- ed with WuT 3,A few scattered cells in germinal center and mantle zone were also stained with WuT3.Cortical thymocyes and some of medullary thymacytes were stained with WuT 4 and WuT8.The numbers of WuT 8 positive mudullary thymocytes were much fewer than that of WuT4.The distribution of WuT 4 and WuT 8 positive Iymphocytes in peripheral lymphoid'tissues were simolar to that of WuT3,but with fewer numbers.When sta- ined with WuT9,26% of bone marrow cells,a few scattered cortical thymocytes and the bottom cells of intestinal mucosa glands were positive.WuTac stained with only a few medullary thym- ocytes.All of the above 5 MAbs for therapeutical use basically showed negative reactions with other non lymphotic tissues and cells.

关 键 词：单克隆抗体 T 淋巴细胞分化抗原 免疫组化酶染色 免疫细胞荧光染色 

分 类 号：R977[医药卫生—药品]