一种新的鼠源性抗人免疫球蛋白多克隆抗体的制备法  

A New Method of Mouse Second Antibodies Preparation

作　　者：尹红章[1] 尹一鸣[1] 张孝月 程明[1] 

机构地区：[1]卫生部北京生物制品研究所,北京100024

出　　处：《中国生物制品学杂志》1991年第4期161-163,共3页Chinese Journal of Biologicals

摘　　要：应用小鼠常规免疫的方法,结合小鼠 SP2/0骨髓瘤细胞诱生腹水的原理制备鼠抗人免疫球蛋白(Igs)腹水。每只小鼠可产生腹水5ml,比小鼠血清高10～20倍,抗体滴度略低,但总特异性抗体的活性高于血清5～10倍。为应用鼠单抗进行免疫诊断提供了制备抗人 Ig 的实用方法,并可用于制备各种鼠源性多克隆抗体。Polyclonal antibodies from mouse ascites can be produced on the basis routine immunization of BALB/c mice and the injection of the mouse tumor cell line(Sp2/0).5 to 10 ml ascites can be obtained from each mouse.The yield by volume is 10—20 fold in comparison with the antiserum.The ELISA titer of the ascitic Abs activity(TA=ELISA titer X volume of ascites or sera)is 5—10 times higher than that of serum Abs activity.It is a new way to produ- ce mouse anti-human-globulin as a seeond Abs used in mouse McAb immunoassy without cross reactivity caused by other second anti-bodies(such as goat anti-human-globulin)and this method can also be applied to prepare other specific Abs in reasonable amount.

关 键 词：人r—球蛋白 免疫腹水 ELISA 

分 类 号：R977[医药卫生—药品]